GRPEL1 抗体 (AA 90-217)
Quick Overview for GRPEL1 抗体 (AA 90-217) (ABIN7602643)
抗原
See all GRPEL1 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 90-217
-
原理
- Anti-GRPEL1 Antibody Picoband®
-
交叉反应 （详细）
- No cross-reactivity with other proteins.
-
产品特性
- Anti-GRPEL1 Antibody Picoband® (ABIN7602643). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
-
纯化方法
- Immunogen affinity purified.
-
免疫原
- E.coli-derived human GRPEL1 recombinant protein (Position: Q90-A217).
-
亚型
- IgG
-
-
anti-GrpE-Like 1, Mitochondrial (GRPEL1) (AA 1-217) antibody
GRPEL1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-GrpE-Like 1, Mitochondrial (GRPEL1) (AA 1-217) antibodyGRPEL1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-GrpE-Like 1, Mitochondrial (GRPEL1) (AA 118-217) antibodyGRPEL1 适用: 人 ELISA 宿主: 小鼠 Monoclonal 3F10 unconjugated
anti-GrpE-Like 1, Mitochondrial (GRPEL1) (AA 35-84) antibodyGRPEL1 适用: 人, 猴 WB 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Choglay, A. A., Chapple, J. P., Blatch, G. L., Cheetham, M. E. Identification and characterization of a human mitochondrial homologue of the bacterial co-chaperone GrpE. Gene 267: 125-134, 2001. 2. Konovalova, S., Liu, X., Manjunath, P., Baral, S., Neupane, N., Hilander, T., Yang, Y., Balboa, D., Terzioglu, M., Euro, L., Varjosalo, M., Tyynismaa, H. Redox regulation of GRPEL2 nucleotide exchange factor for mitochondrial HSP70 chaperone. Redox Biol. 19: 37-45, 2018. 3. Naylor, D. J., Stines, A. P., Hoogenraad, N. J., Hoj, P. B. Evidence for the existence of distinct mammalian cytosolic, microsomal, and two mitochondrial GrpE-like proteins, the co-chaperones of specific Hsp70 members. J. Biol. Chem. 273: 21169-21177, 1998. -
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
-
浓度
- 500 μg/mL
-
缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
-
储存条件
- 4 °C,-20 °C
-
储存方法
-
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
-
-
- GRPEL1 (GrpE-Like 1, Mitochondrial (GRPEL1))
-
别名
- GRPEL1
-
背景
-
Synonyms: Mediator of RNA polymerase II transcription subunit 9, Mediator complex subunit 9, MED9, MED25,
Tissue Specificity: Expressed in fetal brain, fetal lung, fetal liver, heart, brain, placenta, lung, liver, muscle, kidney and pancreas.
Background: Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. Seems to control the nucleotide-dependent binding of mitochondrial HSP70 to substrate proteins.
-
分子量
- 24 kDa
-
基因ID
- 80273
-
途径
- SARS-CoV-2 Protein Interactome
抗原
-
