Nebulette 抗体 (AA 86-842)
Quick Overview for Nebulette 抗体 (AA 86-842) (ABIN7602577)
抗原
See all Nebulette (NEBL) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 86-842
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原理
- Anti-NEBL Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-NEBL Antibody Picoband® (ABIN7602577). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human NEBL recombinant protein (Position: E86-K842).
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亚型
- IgG
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anti-Nebulette (NEBL) antibody
NEBL 适用: 小鼠, 大鼠 WB, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-Nebulette (NEBL) (AA 360-403) antibodyNEBL 适用: 人, 小鼠, 大鼠 WB, IF (p), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Nebulette (NEBL) (AA 438-533) antibodyNEBL 适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.25 μg/mL, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat
ELISA, 0.1-0.5 μg/mL, -
1. Arimura, T., Nakamura, T., Hiroi, S., Satoh, M., Takahashi, M., Ohbuchi, N., Ueda, K., Nouchi, T., Yamaguchi, N., Akai, J., Matsumori, A., Sasayama, S., Kimura, A. Characterization of the human nebulette gene: a polymorphism in an actin-binding motif is associated with nonfamilial idiopathic dilated cardiomyopathy. Hum. Genet. 107: 440-451, 2000. 2. Li, B., Zhuang, L., Trueb, B. Zyxin interacts with the SH3 domains of the cytoskeletal proteins LIM-nebulette and Lasp-1. J. Biol. Chem. 279: 20401-20410, 2004. 3. Millevoi, S., Trombitas, K., Kolmerer, B., Kostin, S., Schaper, J., Pelin, K., Granzier, H., Labeit, S. Characterization of nebulette and nebulin and emerging concepts of their roles for vertebrate Z-discs. J. Molec. Biol. 282: 111-123, 1998. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- Nebulette (NEBL)
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别名
- NEBL
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背景
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Synonyms: Protein phosphatase 1E, Ca (2+)/calmodulin-dependent protein kinase phosphatase N, CaMKP-N, CaMKP-nucleus, CaMKN, Partner of PIX 1, Partner of PIX-alpha, Partner of PIXA, PPM1E, CAMKN, KIAA1072, POPX1
Tissue Specificity: Expressed in fetal and adult brain. Also detected in fetal liver and skeletal muscle, but not in their adult counterparts.
Background: Nebulette is a cardiac-specific isoform belonging to the nebulin family of proteins. It is encoded by the NEBL gene. This gene encodes a nebulin like protein that is abundantly expressed in cardiac muscle. The encoded protein binds actin and interacts with thin filaments and Z-line associated proteins in striated muscle. This protein may be involved in cardiac myofibril assembly. A shorter isoform of this protein termed LIM nebulette is expressed in non-muscle cells and may function as a component of focal adhesion complexes. Alternate splicing results in multiple transcript variants.
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分子量
- 126 kDa
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基因ID
- 10529
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UniProt
- O76041
抗原
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