SMCR8 抗体 (AA 86-776)
Quick Overview for SMCR8 抗体 (AA 86-776) (ABIN7602575)
抗原
See all SMCR8 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 86-776
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原理
- Anti-SMCR8 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-SMCR8 Antibody Picoband® (ABIN7602575). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human SMCR8 recombinant protein (Position: D86-D776).
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亚型
- IgG
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anti-Smith-Magenis Syndrome Chromosome Region, Candidate 8 (SMCR8) (C-Term) antibody
SMCR8 适用: 人, 犬, 马, 兔, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Amick, J., Roczniak-Ferguson, A., Ferguson, S. M. C9orf72 binds SMCR8, localizes to lysosomes, and regulates mTORC1 signaling. Molec. Biol. Cell 27: 3040-3051, 2016. 2. Bi, W., Yan, J., Stankiewicz, P., Park, S.-S., Walz, K., Boerkoel, C. F., Potocki, L., Shaffer, L. G., Devriendt, K., Nowaczyk, M. J. M., Inoue, K., Lupski, J. R. Genes in a refined Smith-Magenis syndrome critical deletion interval on chromosome 17p11.2 and the syntenic region of the mouse. Genome Res. 12: 713-728, 2002. 3. Jung, J., Nayak, A., Schaeffer, V., Starzetz, T., Kirsch, A. K., Muller, S., Dikic, I., Mittelbronn, M., Behrends, C. Multiplex image-based autophagy RNAi screening identifies SMCR8 as ULK1 kinase activity and gene expression regulator. eLife 6: e23063, 2017. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- SMCR8 (Smith-Magenis Syndrome Chromosome Region, Candidate 8 (SMCR8))
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别名
- SMCR8
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背景
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Synonyms: RNA-binding protein 47,RNA-binding motif protein 47,RBM47,
Tissue Specificity: Abundantly expressed in tonsil, lymph node, and trachea, strong expression in prostate, lower expression in thyroid, stomach, and colon. .
Background: Enables protein kinase binding activity and protein kinase inhibitor activity. Contributes to guanyl-nucleotide exchange factor activity. Involved in negative regulation of macromolecule metabolic process, regulation of TOR signaling, and regulation of macroautophagy. Located in chromatin, cytoplasm, and nucleoplasm. Part of guanyl-nucleotide exchange factor complex. Colocalizes with Atg1/ULK1 kinase complex.
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分子量
- 140 kDa
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基因ID
- 140775
抗原
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