NYS48/HAUS8 抗体 (AA 86-322)
Quick Overview for NYS48/HAUS8 抗体 (AA 86-322) (ABIN7602570)
抗原
See all NYS48/HAUS8 (HAUS8) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 86-322
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原理
- Anti-HAUS8 Antibody Picoband®
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交叉反应 (详细)
- No cross-reactivity with other proteins
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产品特性
- Anti-HAUS8 Antibody Picoband® (ABIN7602570). Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human HAUS8 recombinant protein (Position: D86-A322). Human HAUS8 shares r54.1% amino acid (aa) sequence identity with mouse HAUS8.
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亚型
- IgG
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL
1. Goshima, G., Mayer, M., Zhang, N., Stuurman, N., Vale, R. D. Augmin: a protein complex required for centrosome-independent microtubule generation within the spindle. J. Cell Biol. 181: 421-429, 2008. 2. Lawo, S., Bashkurov, M., Mullin, M., Gomez Ferreria, M., Kittler, R., Habermann, B., Tagliaferro, A., Poser, I., Hutchins, J. R. A., Hegemann, B., Pinchev, D., Buchholz, F., Peters, J.-M., Hyman, A. A., Gingras, A.-C., Pelletier, L. HAUS, the 8-subunit human augmin complex, regulates centrosome and spindle integrity. Curr. Biol. 19: 816-826, 2009. 3. Uehara, R., Nozawa, R., Tomioka, A., Petry, S., Vale, R. D., Obuse, C., Goshima, G. The augmin complex plays a critical role in spindle microtubule generation for mitotic progression and cytokinesis in human cells. Proc. Nat. Acad. Sci. 106: 6998-7003, 2009. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- NYS48/HAUS8 (HAUS8) (HAUS Augmin-Like Complex, Subunit 8 (HAUS8))
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别名
- HAUS8
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背景
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Synonyms: HAUS8, HICE1, HAUS augmin-like complex subunit 8, HEC1/NDC80-interacting centrosome-associated protein 1, Sarcoma antigen NY-SAR-48
Background: HAUS8 is 1 of 8 subunits of the 390-kD human augmin complex, or HAUS complex. The augmin complex was first identified in Drosophila, and its name comes from the Latin verb 'augmentare,' meaning 'to increase.' The augmin complex is a microtubule-binding complex involved in microtubule generation within the mitotic spindle and is vital to mitotic spindle assembly.
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分子量
- 45 kDa
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基因ID
- 93323
抗原
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