TUFM 抗体 (AA 8-271)
Quick Overview for TUFM 抗体 (AA 8-271) (ABIN7602475)
抗原
See all TUFM (Tufm) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
 - AA 8-271
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原理
 - Anti-TUFM Antibody Picoband®
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交叉反应 (详细)
 - No cross-reactivity with other proteins.
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产品特性
 - Anti-TUFM Antibody Picoband® (ABIN7602475). Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
 - Immunogen affinity purified.
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免疫原
 - E.coli-derived human TUFM recombinant protein (Position: R8-R271).
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亚型
 - IgG
 
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应用备注
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                        Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. Bogenhagen, D. F., Rousseau, D., Burke, S. The layered structure of human mitochondrial DNA nucleoids. J. Biol. Chem. 283: 3665-3675, 2008. 2. Kohda, M., Tokuzawa, Y., Kishita, Y., Nyuzuki, H., Moriyama, Y., Mizuno, Y., Hirata, T., Yatsuka, Y., Yamashita-Sugahara, Y., Nakachi, Y., Kato, H., Okuda, A., and 23 others. A comprehensive genomic analysis reveals the genetic landscape of mitochondrial respiratory chain complex deficiencies. PLoS Genet. 12: e1005679, 2016. Note: Electronic Article. 3. Liljas, A., Ehrenberg, M., Aqvist, J. Comment on 'The mechanism for activation of GTP hydrolysis on the ribosome.' Science 333: 37 only, 2011. - 
                                            
限制
 - 仅限研究用
 
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状态
 - Lyophilized
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溶解方式
 - Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
 - 500 μg/mL
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缓冲液
 - Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
 - 4 °C,-20 °C
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储存方法
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                        At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. 
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing. 
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- TUFM (Tufm) (Tu Translation Elongation Factor, Mitochondrial (Tufm))
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别名
 - TUFM
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背景
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Synonyms: Phosphopantothenate--cysteine ligase, Phosphopantothenoylcysteine synthetase, PPC synthetase, PPCS, COAB
Tissue Specificity: Expressed in heart, placenta, skeletal muscle kidney, pancreas, spleen, prostate, testis, ovary, ileum and colon. Expressed in lung endothelial and smooth muscle cells (at protein level).
Background: Elongation factor Tu, mitochondrial is a protein that in humans is encoded by the TUFM gene. It is an EF-Tu homolog. This gene encodes a protein which participates in protein translation in mitochondria. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency resulting in lactic acidosis and fatal encephalopathy. A pseudogene has been identified on chromosome 17.
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分子量
 - 50 kDa
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基因ID
 - 7284
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UniProt
 - P49411
 
抗原
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