THEMIS 抗体 (AA 74-588)
Quick Overview for THEMIS 抗体 (AA 74-588) (ABIN7602405)
抗原
See all THEMIS 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 74-588
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原理
- Anti-THEMIS Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-THEMIS Antibody Picoband® (ABIN7602405). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human THEMIS recombinant protein (Position: Q74-H588).
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亚型
- IgG
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anti-Thymocyte Selection Associated (THEMIS) (AA 301-400) antibody
THEMIS 适用: 人, 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro), ICC 宿主: 兔 Polyclonal unconjugated
anti-Thymocyte Selection Associated (THEMIS) (AA 547-641) antibodyTHEMIS 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Thymocyte Selection Associated (THEMIS) (C-Term) antibodyVerified THEMIS 适用: 小鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Thymocyte Selection Associated (THEMIS) (C-Term) antibodyTHEMIS 适用: 人, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Thymocyte Selection Associated (THEMIS) (AA 559-588), (C-Term) antibodyTHEMIS 适用: 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB28989 unconjugated
anti-Thymocyte Selection Associated (THEMIS) (AA 566-595), (C-Term) antibodyTHEMIS 适用: 人, 小鼠 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Fu, G., Casas, J., Rigaud, S., Rybakin, V., Lambolez, F., Brzostek, J., Hoerter, J. A. H., Paster, W., Acuto, O., Cheroutre, H., Sauer, K., Gascoigne, N. R. J. Themis sets the signal threshold for positive and negative selection in T-cell development. Nature 504: 441-445, 2013. 2. Fu, G., Vallee, S., Rybakin, V., McGuire, M. V., Ampudia, J., Brockmeyer, C., Salek, M., Fallen, P. R., Hoerter, J. A. H., Munshi, A., Huang, Y. H., Hu, J., Fox, H. S., Sauer, K., Acuto, O., Gascoigne, N. R. J. Themis controls thymocyte selection through regulation of T cell antigen receptor-mediated signaling. Nature Immun. 10: 848-856, 2009. 3. Johnson, A. L., Aravind, L., Shulzhenko, N., Morgun, A., Choi, S.-Y., Crockford, T. L., Lambe, T., Domaschenz, H., Kucharska, E. M., Zheng, L., Vinuesa, C. G., Lenardo, M. J., Goodnow, C. C., Cornall, R. J., Schwartz, R. H. Themis is a member of a new metazoan gene family and is required for the completion of thymocyte positive selection. Nature Immun. 10: 831-839, 2009. Note: Erratum: Nature Immun. 11: 97 only, 2010. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- THEMIS (Thymocyte Selection Associated (THEMIS))
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别名
- THEMIS
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背景
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Synonyms: Fascin-2, Retinal fascin, FSCN2
Tissue Specificity: Localized specifically in the outer and inner segments of the photoreceptor cells in the retina.
Background: This gene encodes a protein that plays a regulatory role in both positive and negative T-cell selection during late thymocyte development. The protein functions through T-cell antigen receptor signaling, and is necessary for proper lineage commitment and maturation of T-cells. Alternative splicing results in multiple transcript variants.
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分子量
- 73 kDa
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基因ID
- 387357
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UniProt
- Q8N1K5
抗原
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