SPAG5 抗体 (AA 738-1193)
Quick Overview for SPAG5 抗体 (AA 738-1193) (ABIN7602399)
抗原
See all SPAG5 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 738-1193
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原理
- Anti-Astrin/Deepest/SPAG5 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-Astrin/Deepest/SPAG5 Antibody Picoband® (ABIN7602399). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human Astrin/Deepest/SPAG5 recombinant protein (Position: E738-S1193).
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亚型
- IgG
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anti-Sperm Associated Antigen 5 (SPAG5) (AA 1101-1193) antibody
SPAG5 适用: 大鼠 ELISA, IF (cc), IF (p), IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Sperm Associated Antigen 5 (SPAG5) (AA 1-300) antibodySPAG5 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Sperm Associated Antigen 5 (SPAG5) (AA 1082-1191) antibodySPAG5 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 5F9 unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Chang, M.-S., Huang, C.-J., Chen, M.-L., Chen, S.-T., Fan, C.-C., Chu, J.-M., Lin, W.-C., Yang, Y.-C. Cloning and characterization of hMAP126, a new member of mitotic spindle-associated proteins. Biochem. Biophys. Res. Commun. 287: 116-121, 2001. 2. Cheng, T.-S., Hsiao, Y.-L., Lin, C.-C., Yu, C.-T. R, Hsu, C.-M., Chang, M.-S., Lee, C.-I., Huang, C.-Y. F., Howng, S.-L., Hong, Y.-R. Glycogen synthase kinase 3-beta interacts with and phosphorylates the spindle-associated protein astrin. J. Biol. Chem. 283: 2454-2464, 2008. 3. Du, J., Jablonski, S., Yen, T. J., Hannon, G. J. Astrin regulates Aurora-A localization. Biochem. Biophys. Res. Commun. 370: 213-219, 2008. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- SPAG5 (Sperm Associated Antigen 5 (SPAG5))
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别名
- SPAG5
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背景
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Synonyms: Mimecan, Osteoglycin, Ogn, Og
Tissue Specificity: Expressed in kidney.
Background: Sperm-associated antigen 5 is a protein that in humans is encoded by the SPAG5 gene. This gene encodes a protein associated with the mitotic spindle apparatus. The encoded protein may be involved in the functional and dynamic regulation of mitotic spindles.
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分子量
- 130 kDa, 150 kDa
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基因ID
- 10615
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UniProt
- Q96R06
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途径
- M Phase
抗原
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