NOL10 抗体 (AA 73-497)
Quick Overview for NOL10 抗体 (AA 73-497) (ABIN7602390)
抗原
适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 73-497
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原理
- Anti-NOL10 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-NOL10 Antibody Picoband® (ABIN7602390). Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human NOL10 recombinant protein (Position: Y73-D497).
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亚型
- IgG
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anti-Nucleolar Protein 10 (NOL10) (C-Term) antibody
NOL10 适用: 人 WB, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Nucleolar Protein 10 (NOL10) (C-Term) antibodyNOL10 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Nucleolar Protein 10 (NOL10) (AA 300-400) antibodyNOL10 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Nucleolar Protein 10 (NOL10) (C-Term) antibodyNOL10 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, Pig, 兔, Saccharomyces cerevisiae WB 宿主: 兔 Polyclonal unconjugated
anti-Nucleolar Protein 10 (NOL10) (AA 639-688) antibodyNOL10 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. Hartz, P. A. Personal Communication. Baltimore, Md. 1/20/2015. 2. Wada, K., Sato, M., Araki, N., Kumeta, M., Hirai, Y., Takeyasu, K., Furukawa, K., Horigome, T. Dynamics of WD-repeat containing proteins in SSU processome components. Biochem. Cell Biol. 92: 191-199, 2014. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- NOL10 (Nucleolar Protein 10 (NOL10))
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别名
- NOL10
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背景
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Synonyms: RNA-binding protein 47,RNA-binding motif protein 47,RBM47,
Tissue Specificity: Abundantly expressed in tonsil, lymph node, and trachea, strong expression in prostate, lower expression in thyroid, stomach, and colon. .
Background: By immunoprecipitation of human AATF we discovered that AATF forms a salt-stable protein complex together with neuroguidin (NGDN) and NOL10, and demonstrate that the AATF-NGDN-NOL10 (ANN) complex functions in ribosome biogenesis. All three ANN complex members localize to nucleoli and display a mutual dependence with respect to protein stability. Mapping of protein-protein interaction domains revealed the importance of both the evolutionary conserved WD40 repeats in NOL10 and the UTP3/SAS10 domain in NGDN for complex formation.
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分子量
- 80 kDa
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基因ID
- 79954
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途径
- SARS-CoV-2 Protein Interactome
抗原
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