Alanine--tRNA Ligase, Mitochondrial (AARS-1) (AA 729-968) 抗体
Quick Overview for Alanine--tRNA Ligase, Mitochondrial (AARS-1) (AA 729-968) 抗体 (ABIN7602380)
抗原
适用
宿主
克隆类型
应用范围
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抗原表位
- AA 729-968
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原理
- Anti-AlaRS/AARS1 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-AlaRS/AARS1 Antibody Picoband® (ABIN7602380). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human AlaRS/AARS1 recombinant protein (Position: R729-N968).
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亚型
- IgG
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Chihade, J. W., Brown, J. R., Schimmel, P. R., Ribas de Pouplana, L. Origin of mitochondria in relation to evolutionary history of eukaryotic alanyl-tRNA synthetase. Proc. Nat. Acad. Sci. 97: 12153-12157, 2000. 2. Guo, M., Chong, Y. E., Beebe, K., Shapiro, R., Yang, X.-L., Schimmel, P. The C-Ala domain brings together editing and aminoacylation functions on one tRNA. Science 325: 744-747, 2009. Note: Erratum: Science 326: 46 only, 2009. 3. Guo, M., Chong, Y. E., Shapiro, R., Beebe, K., Yang, X.-L., Schimmel, P. Paradox of mistranslation of serine for alanine caused by AlaRS recognition dilemma. Nature 462: 808-812, 2009. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- Alanine--tRNA Ligase, Mitochondrial (AARS-1)
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别名
- AARS1
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背景
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Synonyms: Fos-related antigen 1, FRA-1, FOSL1, FRA1
Tissue Specificity: Adipocytes.
Background: An aminoacyl-tRNA synthetase (aaRS or ARS), also called tRNA-ligase, is an enzyme that attaches the appropriate amino acid onto its corresponding tRNA. The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, of the class II enzymes. Class II tRNA synthases evolved early in evolution and are highly conserved. This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41 % identity witht the E.coli protein. tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure.
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分子量
- 107 kDa
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基因ID
- 16
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UniProt
- P49588
抗原
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