TRMT61B 抗体 (AA 7-477)
Quick Overview for TRMT61B 抗体 (AA 7-477) (ABIN7602334)
抗原
适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 7-477
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原理
- Anti-TRMT61B Antibody Picoband®
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交叉反应 (详细)
- No cross-reactivity with other proteins.
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产品特性
- Anti-TRMT61B Antibody Picoband® (ABIN7602334). Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human TRMT61B recombinant protein (Position: R7-N477).
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亚型
- IgG
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Bar-Yaacov, D., Frumkin, I., Yashiro, I., Chujo, T., Ishigami, Y., Chemla, Y., Blumberg, A., Schlesinger, O., Bieri, P., Greber, B., Ban, N., Zarivach, R., Alfonta, L., Pilpel, Y., Suzuki, T., Mishmar, D. Mitochondrial 16S rRNA is methylated by tRNA methyltransferase TRMT61B in all vertebrates. PLoS Biol. 14: e1002557, 2016. Note: Erratum: PLoS Biol. 15: e1002594, 2017. 2. Chujo, T., Suzuki, T. Trmt61B is a methyltransferase responsible for 1-methyladenosine at position 58 of human mitochondrial tRNAs. RNA 18: 2269-2276, 2012. 3. Gross, M. B. Personal Communication. Baltimore, Md. 6/24/2021. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- TRMT61B (tRNA Methyltransferase 61 (TRMT61B))
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别名
- TRMT61B
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背景
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Synonyms: Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/I, Histone H3/j, Histone H3/k, Histone H3/l, HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, H3FJ
Tissue Specificity: Expressed in fetal brain, fetal lung, fetal liver, heart, brain, placenta, lung, liver, muscle, kidney and pancreas.
Background: Enables mRNA (adenine-N1-)-methyltransferase activity, rRNA (adenine) methyltransferase activity, and tRNA (adenine-N1-)-methyltransferase activity. Involved in mRNA methylation, mitochondrial tRNA methylation, and protein homooligomerization. Located in mitochondrion.
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分子量
- 57 kDa
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基因ID
- 55006
抗原
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