Ribonuclease H1 抗体 (AA 7-223)
Quick Overview for Ribonuclease H1 抗体 (AA 7-223) (ABIN7602317)
抗原
See all Ribonuclease H1 (RNASEH1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 7-223
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原理
- Anti-RNASEH1 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-RNASEH1 Antibody Picoband® (ABIN7602317). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human RNASEH1 recombinant protein (Position: L7-Q223).
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亚型
- IgG
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anti-Ribonuclease H1 (RNASEH1) (AA 189-286) antibody
RNASEH1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 5D10 unconjugated
anti-Ribonuclease H1 (RNASEH1) (AA 73-185) antibodyRNASEH1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ribonuclease H1 (RNASEH1) (Internal Region) antibodyRNASEH1 适用: 人, 小鼠, 大鼠 WB, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Ribonuclease H1 (RNASEH1) (Middle Region) antibodyRNASEH1 适用: 人, 小鼠, 大鼠, Cow, 犬, 马, 兔, 豚鼠, Pig, Saccharomyces cerevisiae, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
anti-Ribonuclease H1 (RNASEH1) (AA 189-286) antibodyRNASEH1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Ribonuclease H1 (RNASEH1) (C-Term) antibodyRNASEH1 适用: 人, 小鼠, 大鼠, Cow, 犬, 马, 兔, Hamster WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Ribonuclease H1 (RNASEH1) (AA 1-286) antibodyRNASEH1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Ribonuclease H1 (RNASEH1) antibodyRNASEH1 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human, Mouse
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Cerritelli, S. M., Crouch, R. J. Cloning, expression, and mapping of ribonucleases H of human and mouse related to bacterial RNase H1. Genomics 53: 300-307, 1998. 2. Cerritelli, S. M., Frolova, E. G., Feng, C., Grinberg, A., Love, P. E., Crouch, R. J. Failure to produce mitochondrial DNA results in embryonic lethality in Rnaseh1 null mice. Molec. Cell 11: 807-815, 2003. 3. Nowotny, M., Gaidamakov, S. A., Ghirlando, R., Cerritelli, S. M., Crouch, R. J., Yang, W. Structure of human RNase H1 complexed with an RNA/DNA hybrid: insight into HIV reverse transcription. Molec. Cell 28: 264-276, 2007. Note: Erratum: Molec. Cell 28: 513 only, 2007. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- Ribonuclease H1 (RNASEH1)
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别名
- RNASEH1
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背景
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Synonyms: Spindle and kinetochore-associated protein 2, Protein FAM33A, SKA2, FAM33A
Background: Ribonuclease H1 also known as RNase H1 is an enzyme that in humans is encoded by the RNASEH1 gene. This gene encodes an endonuclease that specifically degrades the RNA of RNA-DNA hybrids and plays a key role in DNA replication and repair. Alternate in-frame start codon initiation results in the production of alternate isoforms that are ed to the mitochondria or to the nucleus. The production of the mitochondrial isoform is modulated by an upstream open reading frame (uORF). Mutations in this gene have been found in individuals with progressive external ophthalmoplegia with mitochondrial DNA deletions, autosomal recessive 2. Alternative splicing results in additional coding and non-coding transcript variants. Pseudogenes of this gene have been defined on chromosomes 2 and 17.
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分子量
- 38 kDa
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基因ID
- 246243
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UniProt
- O60930
抗原
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