OGFOD3 抗体 (AA 67-311)
This anti-OGFOD3 antibody is a 兔 多克隆 antibody detecting OGFOD3 in WB, ELISA, FACS, ICC 和 IF. Suitable for 人, 小鼠 和 大鼠.
Quick Overview for OGFOD3 抗体 (AA 67-311) (ABIN7602266)
抗原
OGFOD3
(2-Oxoglutarate and Iron-Dependent Oxygenase Domain Containing 3 (OGFOD3))
适用
人, 小鼠, 大鼠
宿主
兔
克隆类型
多克隆
标记
This OGFOD3 antibody is un-conjugated
应用范围
Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
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抗原表位
- AA 67-311
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原理
- Anti-OGFOD3 Antibody Picoband®
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产品特性
- Anti-OGFOD3 Antibody Picoband® (ABIN7602266). Tested in WB, ICC/IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human OGFOD3 recombinant protein (Position: D67-H311). Human OGFOD3 shares 92.2% and 90.6% amino acid (aa) sequence identity with mouse and rat OGFOD3, respectively.
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anti-2-Oxoglutarate and Iron-Dependent Oxygenase Domain Containing 3 (OGFOD3) (AA 95-144) antibody
OGFOD3 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-2-Oxoglutarate and Iron-Dependent Oxygenase Domain Containing 3 (OGFOD3) (N-Term) antibodyOGFOD3 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 兔, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Wang, S., Singh, S. K., Katika, M. R., Lopez-Aviles, S., & Hurtado, A. (2018). High throughput chemical screening reveals multiple regulatory proteins on FOXA1 in breast cancer cell lines. International Journal of Molecular Sciences, 19(12), 4123. 2. Maio, N., Kim, K. S., Singh, A., & Rouault, T. A. (2017). A single adaptable cochaperone-scaffold complex delivers nascent iron-sulfur clusters to mammalian respiratory chain complexes I-III. Cell metabolism, 25(4), 945-953. 3. Liu, X., Salokas, K., Tamene, F., Jiu, Y., Weldatsadik, R. G., 79hman, T., & Varjosalo, M. (2018). An AP-MS-and BioID-compatible MAC-tag enables comprehensive mapping of protein interactions and subcellular localizations. Nature communications, 9(1), 1188. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- OGFOD3 (2-Oxoglutarate and Iron-Dependent Oxygenase Domain Containing 3 (OGFOD3))
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别名
- OGFOD3
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背景
- OGFOD3 (2-oxoglutarate and iron-dependent oxygenase domain-containing protein 3) is a protein involved in the hydroxylation of transfer RNA (tRNA), specifically targeting tRNA-hypoxanthine modification. This process is crucial for maintaining accurate translation and protein synthesis in cells. OGFOD3 belongs to the family of 2-oxoglutarate (2OG)-dependent oxygenases, which require iron and 2OG as cofactors to catalyze various oxidative reactions. Its role highlights the importance of tRNA modifications in cellular physiology, potentially influencing gene expression and protein function regulation.
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分子量
- 36 kDa
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基因ID
- 79701
抗原
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