UBE2I 抗体 (AA 65-146)
Quick Overview for UBE2I 抗体 (AA 65-146) (ABIN7602225)
抗原
See all UBE2I 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 65-146
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原理
- Anti-UBE2I/UBC9 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-UBE2I/UBC9 Antibody Picoband® (ABIN7602225). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human UBE2I/UBC9 recombinant protein (Position: K65-K146 ).
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亚型
- IgG
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anti-Ubiquitin-Conjugating Enzyme E2I (UBE2I) antibody
UBE2I 适用: 人, 猴 WB, IHC, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 1B10 unconjugated
anti-Ubiquitin-Conjugating Enzyme E2I (UBE2I) (AA 1-158) antibodyUBE2I 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2I (UBE2I) (N-Term) antibodyVerified UBE2I 适用: 人, 大鼠 WB, IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2I (UBE2I) (N-Term) antibodyUBE2I 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2I (UBE2I) (AA 1-30) antibodyUBE2I 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2I (UBE2I) antibodyUBE2I 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2I (UBE2I) (AA 1-30), (N-Term) antibodyUBE2I 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB2811 unconjugated
anti-Ubiquitin-Conjugating Enzyme E2I (UBE2I) (AA 33-158) antibodyUBE2I 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2I (UBE2I) (AA 1-157) antibodyUBE2I 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2I (UBE2I) antibodyUBE2I 适用: 人, 小鼠, 大鼠 WB 宿主: 小鼠 Monoclonal 7A9 unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Shi, Y., Zou, M., Farid, N. R., Paterson, M. C. Association of FHIT (fragile histidine triad), a candidate tumour suppressor gene, with the ubiquitin-conjugating enzyme hUBC9. Biochem. J. 352: 443-448, 2000. 2. Wang, Z.-Y., Qiu, Q.-Q., Seufert, W., Taguchi, T., Testa, J. R., Whitmore, S. A., Callen, D. F., Welsh, D., Shenk, T., Deuel, T. F.Molecular cloning of the cDNA and chromosome localization of the gene for human ubiquitin-conjugating enzyme 9. J. Biol. Chem. 271: 24811-24816, 1996. 3. Watanabe TK, Fujiwara T, Kawai A, Shimizu F, Takami S, Hirano H, Okuno S, Ozaki K, Takeda S, Shimada Y, Nagata M, Takaichi A, Takahashi E, Nakamura Y, Shin S (March 1996). "Cloning, expression, and mapping of UBE2I, a novel gene encoding a human homologue of yeast ubiquitin-conjugating enzymes which are critical for regulating the cell cycle".Cytogenet Cell Genet 72 (1): 86-9. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))
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别名
- UBE2I
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背景
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Synonyms: SUMO-conjugating enzyme UBC9, RING-type E3 SUMO transferase UBC9, SUMO-protein ligase, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin-conjugating enzyme E2 I, Ubiquitin-protein ligase I, p18, UBE2I, UBC9, UBCE9
Tissue Specificity: Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus.
Background: SUMO-conjugating enzyme UBC9 (UBE2I), also called UBC9, is a protein that in humans is encoded by the UBE2I gene. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. It is mapped to 16p13.3. UBC9 could fully complement the mutant phenotype of a yeast ubc9 mutant strain. This gene may play a similar role via interaction with WT1, which is able to impose a block to cell cycle progression in eukaryotic cells. What's more, it could support the growth of yeast ubc9 temperature-sensitive mutants at nonpermissive temperatures, indicating that the gene is a functional homolog of yeast ubc9. UBC9 is specifically associated with FHIT, such as FHIT may be involved in cell cycle control through its interaction with UBC9.
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分子量
- 18 kDa
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基因ID
- 7329
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UniProt
- P63279
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途径
- Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Ubiquitin Proteasome Pathway
抗原
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