ORC1 抗体 (AA 628-861)
Quick Overview for ORC1 抗体 (AA 628-861) (ABIN7602199)
抗原
See all ORC1 (ORC1L) 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 628-861
-
原理
- Anti-ORC1 Antibody Picoband®
-
交叉反应 （详细）
- No cross-reactivity with other proteins.
-
产品特性
- Anti-ORC Antibody Picoband® (ABIN7602199). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
-
纯化方法
- Immunogen affinity purified.
-
免疫原
- E.coli-derived human ORC1 recombinant protein (Position: H628-E861).
-
亚型
- IgG
-
-
anti-Origin Recognition Complex, Subunit 1 (ORC1L) antibody
ORC1L 适用: 人, 大鼠, 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Origin Recognition Complex, Subunit 1 (ORC1L) antibodyORC1L 适用: 人 WB, IP 宿主: 大鼠 Monoclonal 7A7 unconjugated
anti-Origin Recognition Complex, Subunit 1 (ORC1L) (AA 1-110) antibodyORC1L 适用: 人 ELISA, IHC (p) 宿主: 小鼠 Monoclonal 3H1 unconjugated
anti-Origin Recognition Complex, Subunit 1 (ORC1L) (Internal Region) antibodyORC1L 适用: 人 WB, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Origin Recognition Complex, Subunit 1 (ORC1L) (AA 1-110) antibodyORC1L 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Origin Recognition Complex, Subunit 1 (ORC1L) antibodyORC1L 适用: 人 ELISA, Coat 宿主: 小鼠 Monoclonal 7F6-1 unconjugated
anti-Origin Recognition Complex, Subunit 1 (ORC1L) (AA 170-220) antibodyORC1L 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Origin Recognition Complex, Subunit 1 (ORC1L) (AA 696-856) antibodyORC1L 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Bicknell, L. S., Bongers, E. M. H. F., Leitch, A., Brown, S., Schoots, J., Harley, M. E., Aftimos, S., Al-Aama, J. Y., Bober, M., Brown, P. A. J., van Bokhoven, H., Dean, J., and 15 others. Mutations in the pre-replication complex cause Meier-Gorlin syndrome. Nature Genet. 43: 356-359, 2011. 2. Bicknell, L. S., Walker, S., Klingseisen, A., Stiff, T., Leitch, A., Kerzendorfer, C., Martin, C.-A., Yeyati, P., Al Sanna, N., Bober, M., Johnson, D., Wise, C., Jackson, A. P., O'Driscoll, M., Jeggo, P. A. Mutations in ORC1, encoding the largest subunit of the origin recognition complex, cause microcephalic primordial dwarfism resembling Meier-Gorlin syndrome. Nature Genet. 43: 350-355, 2011. 3. Bongers, E. M. H. F., Opitz, J. M., Fryer, A., Sarda, P., Hennekam, R. C. M., Hall, B. D., Superneau, D. W., Harbison, M., Poss, A., van Bokhoven, H., Hamel, B. C. J., Knoers, N. V. A. M. Meier-Gorlin syndrome: report of eight additional cases and review. Am. J. Med. Genet. 102: 115-124, 2001. -
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
-
浓度
- 500 μg/mL
-
缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
-
储存条件
- 4 °C,-20 °C
-
储存方法
-
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
-
-
- ORC1 (ORC1L) (Origin Recognition Complex, Subunit 1 (ORC1L))
-
别名
- ORC1
-
背景
-
Synonyms: Muscarinic acetylcholine receptor M2,CHRM2,
Tissue Specificity: Isoform 1 is predominantly expressed in thymus and is also found in peripheral blood leukocytes and kidney. Isoform 2 is predominantly expressed in skeletal muscle and is also found in thymus, kidney, testis, spleen, prostate, ovary, small intestine, heart, placenta and pancreas. Isoform 3 is expressed in thymus and kidney. Isoform 4 is expressed in thymus and skeletal muscle.
Background: Origin recognition complex subunit 1 is a protein that in humans is encoded by the ORC1 gene. The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is the largest subunit of the ORC complex. While other ORC subunits are stable throughout the cell cycle, the levels of this protein vary during the cell cycle, which has been shown to be controlled by ubiquitin-mediated proteolysis after initiation of DNA replication. This protein is found to be selectively phosphorylated during mitosis. It is also reported to interact with MYST histone acetyltransferase 2 (MyST2/HBO1), a protein involved in control of transcription silencing. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
-
分子量
- 100 kDa
-
基因ID
- 4998
-
UniProt
- Q13415
-
途径
- Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA
抗原
-
