SUB1 抗体 (AA 62-127)
Quick Overview for SUB1 抗体 (AA 62-127) (ABIN7602183)
抗原
See all SUB1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
 - AA 62-127
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原理
 - Anti-PC4/SUB1 Antibody Picoband® (monoclonal, 2D13E3)
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交叉反应 (详细)
 - No cross-reactivity with other proteins.
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产品特性
 - Anti-PC4/SUB1 Antibody Picoband® (monoclonal, 2D13E3) (ABIN7602183). Tested in Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
 - Immunogen affinity purified.
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免疫原
 - E.coli-derived human PC4/SUB1 recombinant protein (Position: N62-L127).
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亚型
 - IgG2b
 
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应用备注
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                        Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
1. "Entrez Gene SUB1: SUB1 homolog (S. cerevisiae)". 2. Kretzschmar M, Kaiser K, Lottspeich F, Meisterernst M (August 1994). "A novel mediator of class II gene transcription with homology to viral immediate-early transcriptional regulators". Cell 78 (3): 525-34. 3. Ge H, Roeder RG (August 1994). "Purification, cloning, and characterization of a human coactivator, PC4, that mediates transcriptional activation of class II genes". Cell 78 (3): 513-23. - 
                                            
限制
 - 仅限研究用
 
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状态
 - Lyophilized
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溶解方式
 - Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
 - 500 μg/mL
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缓冲液
 - Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
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储存条件
 - 4 °C,-20 °C
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储存方法
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                        At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. 
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing. 
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- SUB1 (Activated RNA Polymerase II Transcriptional Coactivator p15 (SUB1))
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别名
 - SUB1
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背景
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Synonyms: Alpha-actinin-3, Alpha-actinin skeletal muscle isoform 3, F-actin cross-linking protein, ACTN3
Tissue Specificity: Expressed only in a subset of type 2 skeletal muscle fibers.
Background: Activated RNA polymerase II transcriptional coactivator p15, also known as positive cofactor 4 (PC4) or SUB1 homolog, is a protein that in humans is encoded by the SUB1 gene. This gene is mapped to 5p13.3. The transcriptional cofactor PC4 is an ancient single-strand DNA (ssDNA)-binding protein that has a homologue in bacteriophage T5 where it is likely the elusive replicative ssDNA-binding protein. The recombinant PC4 is shown to function identically to the native protein through its interaction with TAFs.
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分子量
 - 18 kDa
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基因ID
 - 10923
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UniProt
 - P53999
 
抗原
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