WFS1 抗体 (AA 61-313)
Quick Overview for WFS1 抗体 (AA 61-313) (ABIN7602165)
抗原
See all WFS1 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 61-313
-
原理
- Anti-WFS1 Antibody Picoband®
-
交叉反应 （详细）
- No cross-reactivity with other proteins.
-
产品特性
- Anti-WFS1 Antibody Picoband® (ABIN7602165). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
-
纯化方法
- Immunogen affinity purified.
-
免疫原
- E.coli-derived human WFS1 recombinant protein (Position: A61-H313).
-
亚型
- IgG
-
-
anti-Wolfram Syndrome 1 (WFS1) (AA 1-285) antibody
WFS1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Wolfram Syndrome 1 (WFS1) (Internal Region) antibodyWFS1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Wolfram Syndrome 1 (WFS1) (AA 791-890) antibodyWFS1 适用: 人 WB, ELISA, ICC, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Western blot, 0.25-0.5 μg/mL, Human, Monkey
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Berry, V., Gregory-Evans, C., Emmett, W., Waseem, N., Raby, J., Prescott, D., Moore, A. T., Bhattacharya, S. S. Wolfram gene (WFS1) mutation causes autosomal dominant congenital nuclear cataract in humans. Europ. J. Hum. Genet. 21: 1356-1360, 2013. 2. Bespalova, I. N., Van Camp, G., Bom, S. J. H., Brown, D. J., Cryns, K., DeWan, A. T., Erson, A. E., Flothmann, K., Kunst, H. P. M., Kurnool, P., Sivakumaran, T. A., Cremers, C. W. R. J., Leal, S. M., Burmeister, M., Lesperance, M. M. Mutations in the Wolfram syndrome 1 gene (WFS1) are a common cause of low frequency sensorineural hearing loss. Hum. Molec. Genet. 10: 2501-2508, 2001. 3. Colosimo, A., Guida, V., Rigoli, L., Di Bella, C., De Luca, A., Briuglia, S., Stuppia, L., Salpietro, D. C., Dallapiccolo, B. Molecular detection of novel WFS1 mutations in patients with Wolfram syndrome by a DHPLC-based assay. Hum. Mutat. 21: 622-629, 2003. -
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
-
浓度
- 500 μg/mL
-
缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
-
储存条件
- 4 °C,-20 °C
-
储存方法
-
Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
-
-
- WFS1 (Wolfram Syndrome 1 (WFS1))
-
别名
- WFS1
-
背景
-
Synonyms: Wee1-like protein kinase, WEE1hu, Wee1A kinase, WEE1
Tissue Specificity: Predominantly expressed in T-cells. Also detected in proliferating intestinal epithelial cells and in the basal epithelial cells of mammary gland epithelium.
Background: Wolframin is a protein that in humans is encoded by the WFS1 gene. This gene encodes a transmembrane protein, which is located primarily in the endoplasmic reticulum and ubiquitously expressed with highest levels in brain, pancreas, heart, and insulinoma beta-cell lines. Mutations in this gene are associated with Wolfram syndrome, also called DIDMOAD (Diabetes Insipidus, Diabetes Mellitus, Optic Atrophy, and Deafness), an autosomal recessive disorder. The disease affects the brain and central nervous system. Mutations in this gene can also cause autosomal dominant deafness 6 (DFNA6), also known as DFNA14 or DFNA38. Alternatively spliced transcript variants have been found for this gene.
-
分子量
- 100 kDa
-
基因ID
- 7466
-
UniProt
- O76024
-
途径
- Sensory Perception of Sound, Carbohydrate Homeostasis, ER-Nucleus Signaling, Negative Regulation of intrinsic apoptotic Signaling, SARS-CoV-2 Protein Interactome
抗原
-
