CEP250 抗体 (AA 563-853)
Quick Overview for CEP250 抗体 (AA 563-853) (ABIN7602053)
抗原
See all CEP250 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 563-853
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原理
- Anti-CEP250 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-C Antibody Picoband® (ABIN7602053). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human CEP250 recombinant protein (Position: Q563-K853).
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亚型
- IgG
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anti-Centrosomal Protein 250kDa (CEP250) (C-Term) antibody
CEP250 适用: 人, 小鼠 ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-Centrosomal Protein 250kDa (CEP250) (AA 141-240) antibodyCEP250 适用: 人 ELISA, WB 宿主: 小鼠 Monoclonal 4A1 unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Bahe, S., Stierhof, Y.-D., Wilkinson, C. J., Leiss, F., Nigg, E. A. Rootletin forms centriole-associated filaments and functions in centrosome cohesion. J. Cell Biol. 171: 27-33, 2005. 2. de Castro-Miro, M., Tonda, R., Escudero-Ferruz, P., Andres, R., Mayor-Lorenzo, A., Castro, J., Ciccioli, M., Hidalgo, D. A., Rodriguez-Ezcurra, J. J., Farrando, J., Perez-Santonja, J. J., Cormand, B., Marfany, G., Gonzalez-Duarte, R. Novel candidate genes and a wide spectrum of structural and point mutations responsible for inherited retinal dystrophies revealed by exome sequencing. PLoS One 11: e0168966, 2016. Note: Electronic Article. 3. Fry, A. M., Mayor, T., Meraldi, P., Stierhof, Y.-D., Tanaka, K., Nigg, E. A. C-Nap1, a novel centrosomal coiled-coil protein and candidate substrate of the cell cycle-regulated protein kinase Nek2. J. Cell Biol. 141: 1563-1574, 1998. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- CEP250 (Centrosomal Protein 250kDa (CEP250))
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别名
- CEP250
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背景
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Synonyms: Gastrotropin, GT, Fatty acid-binding protein 6, Ileal lipid-binding protein, ILBP, Intestinal 15 kDa protein, I-15P, Intestinal bile acid-binding protein, I-BABP, FABP6, ILBP, ILLBP
Tissue Specificity: Isoform 1 is expressed in the jejunum, ileum, cecum and ascending colon intestine. Isoform 2 is xpressed in the gallbladder, duodenum, jejunum, ileum, cecum, ascending, transverse and descending colon, sigmoid colon and rectum. Isoform 2 is expressed in colorectal adenocarcinomas and their adjacent normal mucosa.
Background: Centrosome-associated protein CEP250 is a protein that in humans is encoded by the CEP250 gene. This gene encodes a core centrosomal protein required for centriole-centriole cohesion during interphase of the cell cycle. The encoded protein dissociates from the centrosomes when parental centrioles separate at the beginning of mitosis. The protein associates with and is phosphorylated by NIMA-related kinase 2, which is also associated with the centrosome. Alternative splicing results in multiple transcript variants encoding different isoforms.
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分子量
- 250 kDa
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基因ID
- 11190
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途径
- M Phase, SARS-CoV-2 Protein Interactome
抗原
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