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MDH1 抗体 (AA 56-334)

This anti-MDH1 antibody is a 兔 多克隆 antibody detecting MDH1 in WB, ELISA 和 FACS. Suitable for 人, 小鼠 和 大鼠.
产品编号 ABIN7602039
发货至: 中国

Quick Overview for MDH1 抗体 (AA 56-334) (ABIN7602039)

抗原

See all MDH1 抗体
MDH1 (Malate Dehydrogenase 1, NAD (Soluble) (MDH1))

适用

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人, 小鼠, 大鼠

宿主

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克隆类型

  • 63
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多克隆

标记

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This MDH1 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Flow Cytometry (FACS)
  • 抗原表位

    • 16
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    AA 56-334

    原理

    Anti-MDH1 Antibody Picoband®

    交叉反应 (详细)

    No cross-reactivity with other proteins.

    产品特性

    Anti-MDH1 Antibody Picoband® (ABIN7602039). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    纯化方法

    Immunogen affinity purified.

    免疫原

    E.coli-derived human MDH1 recombinant protein (Position: E56-A334).

    亚型

    IgG
  • 应用备注

    Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
    ELISA, 0.1-0.5 μg/mL, -
    1. Ball, S. T., Moseley, H. J., Peters, J. Mor2, supernatant malate dehydrogenase, is linked to wa2 and Hba on mouse chromosome 11 in a region of homology with human chromosome 2p. Genomics 24: 399-400, 1994. 2. Birktoft, J. J., Fernley, R. T., Bradshaw, R. A., Banaszak, L. J. Amino acid sequence homology among the 2-hydroxy acid dehydrogenases: mitochondrial and cytoplasmic malate dehydrogenases form a homologous system with lactate dehydrogenase. Proc. Nat. Acad. Sci. 79: 6166-6170, 1982. 3. Blake, N. M., Kirk, R. L., Simons, M. J., Alpers, M. P. Genetic variants of soluble malate dehydrogenase in New Guinea populations. Humangenetik 11: 72-74, 1970.

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    浓度

    500 μg/mL

    缓冲液

    Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

    储存条件

    4 °C,-20 °C

    储存方法

    At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
  • 抗原

    MDH1 (Malate Dehydrogenase 1, NAD (Soluble) (MDH1))

    别名

    MDH1

    背景

    Synonyms: Calretinin, CR, 29 kDa calbindin, CALB2, CAB29

    Tissue Specificity: Brain.

    Background: Malate dehydrogenase, cytoplasmic also known as malate dehydrogenase 1 is an enzyme that in humans is encoded by the MDH1 gene. This gene encodes an enzyme that catalyzes the NAD/NADH-dependent, reversible oxidation of malate to oxaloacetate in many metabolic pathways, including the citric acid cycle. Two main isozymes are known to exist in eukaryotic cells: one is found in the mitochondrial matrix and the other in the cytoplasm. This gene encodes the cytosolic isozyme, which plays a key role in the malate-aspartate shuttle that allows malate to pass through the mitochondrial membrane to be transformed into oxaloacetate for further cellular processes. Alternatively spliced transcript variants have been found for this gene. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Pseudogenes have been identified on chromosomes X and 6.

    分子量

    36 kDa

    基因ID

    4190

    UniProt

    P40925
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