PIWIL4 抗体 (AA 51-749)
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- 抗原 See all PIWIL4 抗体
- PIWIL4 (Piwi-Like 4 (PIWIL4))
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抗原表位
- AA 51-749
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This PIWIL4 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)
- 原理
- Anti-PIWIL4/PIWI Antibody Picoband®
- 交叉反应 （详细）
- No cross-reactivity with other proteins.
- 产品特性
- Anti-PIWIL4/PIWI Antibody Picoband® (ABIN7601940). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- 纯化方法
- Immunogen affinity purified.
- 免疫原
- E.coli-derived human PIWIL4/PIWI recombinant protein (Position: R51-N749).
- 亚型
- IgG
- Top Product
- Discover our top product PIWIL4 Primary Antibody
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anti-Piwi-Like 4 (PIWIL4) (AA 304-434) antibody
PIWIL4 适用: 人 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 10G9B11 unconjugated
anti-Piwi-Like 4 (PIWIL4) (AA 260-460) antibodyPIWIL4 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Piwi-Like 4 (PIWIL4) (AA 12-235) antibodyPIWIL4 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Piwi-Like 4 (PIWIL4) (AA 1-852) antibodyPIWIL4 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Piwi-Like 4 (PIWIL4) antibodyPIWIL4 适用: 人, 小鼠, 大鼠 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-Piwi-Like 4 (PIWIL4) antibodyPIWIL4 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Piwi-Like 4 (PIWIL4) (AA 1-852) antibodyPIWIL4 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Piwi-Like 4 (PIWIL4) (N-Term) antibodyPIWIL4 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Carmell, M. A., Girard, A., van de Kant, H. J. G., Bourc'his, D., Bestor, T. H., de Rooij, D. G., Hannon, G. J. MIWI2 is essential for spermatogenesis and repression of transposons in the mouse male germline. Dev. Cell 12: 503-514, 2007. 2. De Fazio, S., Bartonicek, N., Di Giacomo, M., Abreu-Goodger, C., Sankar, A., Funaya, C., Antony, C., Moreira, P. N., Enright, A. J., O'Carroll, D. The endonuclease activity of Mili fuels piRNA amplification that silences LINE1 elements. Nature 480: 259-263, 2011. 3. Sasaki, T., Shiohama, A., Minoshima, S., Shimizu, N. Identification of eight members of the Argonaute family in the human genome. Genomics 82: 323-330, 2003. - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 浓度
- 500 μg/mL
- 缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- 抗原
- PIWIL4 (Piwi-Like 4 (PIWIL4))
- 别名
- PIWIL4 (PIWIL4 产品)
- 背景
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Synonyms: PR domain zinc finger protein 5, PR domain-containing protein 5, PRDM5, PFM2
Tissue Specificity: Widely expressed with highest levels in colon and ovary. Tends to be silenced in breast, colorectal, gastric and liver cancer tissues.
Background: PIWI-like protein 4 (PIWIL4) is a 97 kDa member of the argonaute family of proteins and the PIWI subfamily. Human PIWIL4 is 852 amino acids (aa) in length and contains one PAZ domain (aa 268-384) and one PIWI domain (aa 546-838). There are three isoforms for human PIWIL4. Isoform 1 is the standard protein. Isoform 2 has a two aa substitution for aa 1-29 in isoform 1 and a deletion of aa 523-852. Isoform 3 has a deletion of aa 1-69 and a deletion of aa 523-852. Human PIWIL4 shares 77 % aa sequence identity with mouse and rat PIWIL4. PIWIL4 plays a central role in spermatogenesis.
- 分子量
- 97 kDa
- 基因ID
- 143689
- UniProt
- Q7Z3Z4
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