PIWIL4 抗体 (AA 51-749)
Quick Overview for PIWIL4 抗体 (AA 51-749) (ABIN7601940)
抗原
See all PIWIL4 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 51-749
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原理
- Anti-PIWIL4/PIWI Antibody
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交叉反应 (详细)
- No cross-reactivity with other proteins.
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产品特性
- Anti-PIWIL4/PIWI Antibody (ABIN7601940). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human PIWIL4/PIWI recombinant protein (Position: R51-N749).
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亚型
- IgG
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- PIWIL4 (Piwi-Like 4 (PIWIL4))
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别名
- PIWIL4
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背景
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Synonyms: PR domain zinc finger protein 5, PR domain-containing protein 5, PRDM5, PFM2
Tissue Specificity: Widely expressed with highest levels in colon and ovary. Tends to be silenced in breast, colorectal, gastric and liver cancer tissues.
Background: PIWI-like protein 4 (PIWIL4) is a 97 kDa member of the argonaute family of proteins and the PIWI subfamily. Human PIWIL4 is 852 amino acids (aa) in length and contains one PAZ domain (aa 268-384) and one PIWI domain (aa 546-838). There are three isoforms for human PIWIL4. Isoform 1 is the standard protein. Isoform 2 has a two aa substitution for aa 1-29 in isoform 1 and a deletion of aa 523-852. Isoform 3 has a deletion of aa 1-69 and a deletion of aa 523-852. Human PIWIL4 shares 77 % aa sequence identity with mouse and rat PIWIL4. PIWIL4 plays a central role in spermatogenesis.
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分子量
- 97 kDa
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基因ID
- 143689
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UniProt
- Q7Z3Z4
抗原
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