Melanoma Antigen Family D, 4 (MAGED4) (AA 51-741) 抗体
Quick Overview for Melanoma Antigen Family D, 4 (MAGED4) (AA 51-741) 抗体 (ABIN7601938)
抗原
See all Melanoma Antigen Family D, 4 (MAGED4) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 51-741
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原理
- Anti-MAGED4 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-MAGED4 Antibody Picoband® (ABIN7601938). Tested in ELISA, IF, IHC, ICC, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human MAGED4 recombinant protein (Position: F51-R741).
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亚型
- IgG
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anti-Melanoma Antigen Family D, 4 (MAGED4) (Center) antibody
MAGED4 适用: 人 WB, ICC, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry, 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Chomez, P., De Backer, O., Bertrand, M., De Plaen, E., Boon, T., Lucas, S. An overview of the MAGE gene family with the identification of all human members of the family. Cancer Res. 61: 5544-5551, 2001. [PubMed: 11454705, related citations] 2. Hartz, P. A. Personal Communication. Baltimore, Md. 4/30/2009. 3. Ito, S., Kawano, Y., Katakura, H., Takenaka, K., Adachi, M., Sasaki, M., Shimizu, K., Ikenaka, K., Wada, H., Tanaka, F. Expression of MAGE-D4, a novel MAGE family antigen, is correlated with tumor-cell proliferation of non-small cell lung cancer. Lung Cancer 51: 79-88, 2006. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- Melanoma Antigen Family D, 4 (MAGED4)
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别名
- MAGED4
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背景
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Synonyms: Kelch repeat and BTB domain-containing protein 2, BTB and kelch domain-containing protein 1, KBTBD2, BKLHD1, KIAA1489
Tissue Specificity: Detected in liver, skeletal muscle, kidney, pancreas, spleen, thyroid, testis, ovary, small intestine and colon.
Background: MAGE family member D4 is a protein that in humans is encoded by the MAGED4 gene. MAGED4, originally termedMAGEE1, is a member ofMAGE (Melanoma associated antigen)super-family identified by serial analysis of gene expression (SAGE) technique (PMID: 11406556, 24966945). Three isoforms(MAGED4a, b, and c) were produced by alternative splice in the process of gene transcription (PMID: 11406556). This antibody raised against 1-350 aa of humanMAGED4 protein detects a band of 90-100 kDa, which islargerthan the calculated molecularweight. The slower electrophoretic mobility may be due to abnormal spatial conformation ofMAGED4 protein caused by abundance of proline in composition (PMID: 24966945).
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分子量
- 100 kDa
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基因ID
- 728239
抗原
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