ECD/SGT1 抗体 (AA 50-567)
Quick Overview for ECD/SGT1 抗体 (AA 50-567) (ABIN7601915)
抗原
See all ECD/SGT1 (ECD) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 50-567
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原理
- Anti-SGT1/ECD Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-SGT1/ECD Antibody Picoband® (ABIN7601915). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human SGT1/ECD recombinant protein (Position: L50-Q567).
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亚型
- IgG
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anti-Ecdysoneless Homolog (ECD) (AA 1-644) antibody
ECD 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1A11 unconjugated
anti-Ecdysoneless Homolog (ECD) (AA 1-300) antibodyECD 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Ecdysoneless Homolog (ECD) (AA 1-644) antibodyECD 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Ecdysoneless Homolog (ECD) (C-Term) antibodyECD 适用: 人, 山羊, 马, Cow, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Ecdysoneless Homolog (ECD) (AA 72-121) antibodyECD 适用: 人, 山羊, 马, 兔, Cow, Pig, 犬, 豚鼠, 猴 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Hartz, P. A. Personal Communication. Baltimore, Md. 7/9/2015. 2. Kim, J. H., Gurumurthy, C. B., Band, H., Band, V. Biochemical characterization of human Ecdysoneless reveals a role in transcriptional regulation. Biol. Chem. 391: 9-19, 2010. 3. Sato, T., Jigami, Y., Suzuki, T., Uemura, H. A human gene, hSGT1, can substitute for GCR2, which encodes a general regulatory factor of glycolytic gene expression in Saccharomyces cerevisiae. Molec. Gen. Genet. 260: 535-540, 1999. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- ECD/SGT1 (ECD) (Ecdysoneless Homolog (ECD))
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别名
- ECD
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背景
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Synonyms: Disintegrin and metalloproteinase domain-containing protein 10, ADAM 10, CDw156, Kuzbanian protein homolog, Mammalian disintegrin-metalloprotease, CD156c, ADAM10, KUZ, MADM
Tissue Specificity: Expressed in spleen, lymph node, thymus, peripheral blood leukocyte, bone marrow, cartilage, chondrocytes and fetal liver.
Background: Protein SGT1 is a protein that in humans is encoded by the ECD gene. Regulator of p53/TP53 stability and function. Inhibits MDM2-mediated degradation of p53/TP53 possibly by cooperating in part with TXNIP. May be involved transcriptional regulation. In vitro has intrinsic transactivation activity enhanced by EP300. May be a transcriptional activator required for the expression of glycolytic genes. Involved in regulation of cell cycle progression. Proposed to disrupt Rb-E2F binding leading to transcriptional activation of E2F proteins. The cell cycle -regulating function may depend on its RUVBL1-mediated association with the R2TP complex. May play a role in regulation of pre-mRNA splicing.
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分子量
- 80 kDa
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基因ID
- 11319
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UniProt
- O95905
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途径
- Regulation of Carbohydrate Metabolic Process
抗原
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