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GOT1 抗体 (AA 5-413)

This anti-GOT1 antibody is a 兔 多克隆 antibody detecting GOT1 in WB, IHC, ELISA, ICC, IF 和 FACS. Suitable for 人, 小鼠 和 大鼠.
产品编号 ABIN7601884
发货至: 中国

Quick Overview for GOT1 抗体 (AA 5-413) (ABIN7601884)

抗原

See all GOT1 抗体
GOT1 (Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1))

适用

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人, 小鼠, 大鼠

宿主

  • 79
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克隆类型

  • 87
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多克隆

标记

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This GOT1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)
  • 抗原表位

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    AA 5-413

    原理

    Anti-Aspartate Aminotransferase/GOT1 Antibody Picoband®

    交叉反应 (详细)

    No cross-reactivity with other proteins.

    产品特性

    Anti-Aspartate Aminotransferase/GOT1 Antibody Picoband® (ABIN7601884). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    纯化方法

    Immunogen affinity purified.

    免疫原

    E.coli-derived human Aspartate Aminotransferase/GOT1 recombinant protein (Position: S5-Q413).

    亚型

    IgG
  • 应用备注

    Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
    Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat
    Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
    ELISA, 0.1-0.5 μg/mL, -
    1. Aitken, D. A., Ferguson-Smith, M. A. Gene dosage evidence for the regional assignment of the GOT-S structural gene locus to 10q24-10q25. Cytogenet. Cell Genet. 22: 468-471, 1978. 2. Creagan, R., Tischfield, J., McMorris, F. A., Chen, S.-H., Hirschi, M., Chen, T.-T., Ricciuti, F., Ruddle, F. H. Assignment of the genes for human peptidase A to chromosome 18 and cytoplasmic glutamic oxaloacetate transaminase to chromosome 10 using somatic-cell hybrids. Cytogenet. Cell Genet. 12: 187-198, 1973. 3. Doonan, S., Barra, D., Bossa, F. Structural and genetic relationships between cytosolic and mitochondrial isoenzymes. Int. J. Biochem. 16: 1193-1199, 1984.

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    浓度

    500 μg/mL

    缓冲液

    Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

    储存条件

    4 °C,-20 °C

    储存方法

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • 抗原

    GOT1 (Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1))

    别名

    GOT1

    背景

    Synonyms: Muscarinic acetylcholine receptor M1, CHRM1

    Tissue Specificity: Sperm. Mainly localized in the tail and in the postacrosomal region but is also found in the midpiece and basal region in a small percentage of sperm cells. Reduced levels found in the sperms of asthenozoospermia and leukocytospermia patients (at protein level). Spleen, lymph nodes, appendix, and fetal liver. Expressed in lymphocytes, T-cells and B-cells but not in natural killer cells, monocytes or granulocytes.

    Background: Aspartate aminotransferase, cytoplasmic is an enzyme that in humans is encoded by the GOT1 gene. Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.

    分子量

    41 kDa

    基因ID

    2805

    UniProt

    P17174

    途径

    Hepatitis C, Monocarboxylic Acid Catabolic Process, Methionine Biosynthetic Process
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