Pleckstrin 抗体 (AA 47-308)
Quick Overview for Pleckstrin 抗体 (AA 47-308) (ABIN7601814)
抗原
See all Pleckstrin (PLEK) 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 47-308
-
原理
- Anti-Pleckstrin/PLEK Antibody Picoband®
-
交叉反应 （详细）
- No cross-reactivity with other proteins.
-
产品特性
- Anti-Pleckstrin/PLEK Antibody Picoband® (ABIN7601814). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
-
纯化方法
- Immunogen affinity purified.
-
免疫原
- E.coli-derived human Pleckstrin/PLEK recombinant protein (Position: M47-K308).
-
亚型
- IgG
-
-
anti-Pleckstrin (PLEK) (AA 121-231) antibody
PLEK 适用: 人 WB, IHC, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 6E3 unconjugated
anti-Pleckstrin (PLEK) (AA 121-230) antibodyPLEK 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 6E3 unconjugated
anti-Pleckstrin (PLEK) (C-Term) antibodyVerified PLEK 适用: 人 WB, IHC, ELISA, FACS, IF 宿主: 山羊 Polyclonal unconjugated
anti-Pleckstrin (PLEK) (AA 121-230) antibodyPLEK 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2D8 unconjugated
anti-Pleckstrin (PLEK) (AA 121-230) antibodyPLEK 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Pleckstrin (PLEK) (AA 1-350) antibodyPLEK 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Pleckstrin (PLEK) (C-Term) antibodyPLEK 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Pleckstrin (PLEK) (N-Term) antibodyPLEK 适用: 人, 小鼠, 大鼠, Cow, 豚鼠, 斑马鱼, Bat, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Pleckstrin (PLEK) (AA 1-350) antibodyPLEK 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Pleckstrin (PLEK) antibodyPLEK 适用: 人 WB 宿主: 兔 Monoclonal unconjugated
-
-
应用备注
-
Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Cmarik, J. L., Hegamyer, G., Gerrard, B., Dean, M., Colburn, N. H. cDNA cloning and mapping of mouse pleckstrin (Plek), a gene upregulated in transformation-resistant cells. Genomics 66: 204-212, 2000. 2. Tyers, M., Haslam, R. J., Rachubinski, R. A., Harley, C. B. Molecular analysis of pleckstrin: the major protein kinase C substrate of platelets. J. Cell. Biochem. 40: 133-145, 1989. 3. Tyers, M., Rachubinski, R. A., Stewart, M. I., Varrichio, A. M., Shorr, R. G. L., Haslam, R. J., Harley, C. B. Molecular cloning and expression of the major protein kinase C substrate of platelets. Nature 333: 470-473, 1988. -
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
-
浓度
- 500 μg/mL
-
缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
-
储存条件
- 4 °C,-20 °C
-
储存方法
-
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
-
-
- Pleckstrin (PLEK)
-
别名
- PLEK
-
背景
-
Synonyms: Ubiquitin carboxyl-terminal hydrolase 21, Deubiquitinating enzyme 21, Ubiquitin thioesterase 21, Ubiquitin-specific-processing protease 21, USP21, USP23, PP1490
Tissue Specificity: Highly expressed in heart, pancreas and skeletal muscle. Also expressed in brain, placenta, liver and kidney, and at very low level in lung.
Background: Enables phosphatidylinositol-3,4-bisphosphate binding activity, protein homodimerization activity, and protein kinase C binding activity. Involved in several processes, including G protein-coupled receptor signaling pathway, actin cytoskeleton organization, and positive regulation of supramolecular fiber organization. Located in cytoplasm and ruffle membrane.
-
分子量
- 40 kDa
-
基因ID
- 5341
-
UniProt
- P08567
-
途径
- Inositol Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process
抗原
-
