DBT 抗体 (AA 46-462)
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北京 101111
Quick Overview for DBT 抗体 (AA 46-462) (ABIN7601800)
抗原
See all DBT 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 46-462
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原理
- Anti-DBT Antibody
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交叉反应 （详细）
- No cross-reactivity with other proteins
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预测反应
- Human DBT shares 89.7% amino acid (aa) sequence identity with mouse DBT.
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产品特性
- Anti-DBT Antibody. Tested in WB, ICC/IF, IP, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human DBT recombinant protein (Position: K46-R462). Human DBT shares 89.7% amino acid (aa) sequence identity with mouse DBT.
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亚型
- IgG
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anti-Dihydrolipoamide Branched Chain Transacylase E2 (DBT) (AA 1-482) antibody
DBT 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Dihydrolipoamide Branched Chain Transacylase E2 (DBT) (AA 35-84) antibodyDBT 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 兔, 猴, Pig, Bat WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Dihydrolipoamide Branched Chain Transacylase E2 (DBT) (AA 1-482) antibodyDBT 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Dihydrolipoamide Branched Chain Transacylase E2 (DBT) (N-Term) antibodyDBT 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Dihydrolipoamide Branched Chain Transacylase E2 (DBT) antibodyDBT 适用: 人 WB, FACS 宿主: 小鼠 Monoclonal 1G2 unconjugated
anti-Dihydrolipoamide Branched Chain Transacylase E2 (DBT) (N-Term) antibodyDBT 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 兔, 斑马鱼, Saccharomyces cerevisiae WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Dihydrolipoamide Branched Chain Transacylase E2 (DBT) (AA 140-259) antibodyDBT 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
- Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Immunoprecipitation, 2-4 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/mL
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing. -
有效期
- 12 months
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- DBT (Dihydrolipoamide Branched Chain Transacylase E2 (DBT))
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别名
- DBT
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背景
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Background: The branched-chain alpha-keto acid dehydrogenase complex (BCKD) is an inner-mitochondrial enzyme complex involved in the breakdown of the branched-chain amino acids isoleucine, leucine, and valine. The BCKD complex is thought to be composed of a core of 24 transacylase (E2) subunits, and associated decarboxylase (E1), dehydrogenase (E3), and regulatory subunits. This gene encodes the transacylase (E2) subunit. Mutations in this gene result in maple syrup urine disease, type 2. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.
Gene Full Name: dihydrolipoamide branched chain transacylase E2
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分子量
- 53 kDa
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基因ID
- 1629
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UniProt
- P11182
抗原
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