DBT 抗体 (AA 46-462)
Quick Overview for DBT 抗体 (AA 46-462) (ABIN7601800)
抗原
See all DBT 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 46-462
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原理
- Anti-DBT Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-DBT Antibody Picoband® (ABIN7601800). Tested in WB, ICC/IF, IP, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human DBT recombinant protein (Position: K46-R462). Human DBT shares 89.7% amino acid (aa) sequence identity with mouse DBT.
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亚型
- IgG
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anti-Dihydrolipoamide Branched Chain Transacylase E2 (DBT) (AA 35-84) antibody
DBT 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 兔, 猴, Pig, Bat WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Dihydrolipoamide Branched Chain Transacylase E2 (DBT) (AA 1-482) antibodyDBT 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Dihydrolipoamide Branched Chain Transacylase E2 (DBT) (AA 1-482) antibodyDBT 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Dihydrolipoamide Branched Chain Transacylase E2 (DBT) (N-Term) antibodyDBT 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Dihydrolipoamide Branched Chain Transacylase E2 (DBT) antibodyDBT 适用: 人 WB, FACS 宿主: 小鼠 Monoclonal 1G2 unconjugated
anti-Dihydrolipoamide Branched Chain Transacylase E2 (DBT) (N-Term) antibodyDBT 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 兔, 斑马鱼, Saccharomyces cerevisiae WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Dihydrolipoamide Branched Chain Transacylase E2 (DBT) (AA 140-259) antibodyDBT 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Immunoprecipitation, 2-4 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL
1. Bogenhagen, D. F., Rousseau, D., Burke, S. The layered structure of human mitochondrial DNA nucleoids. J. Biol. Chem. 283: 3665-3675, 2008. 2. Chi, C.-S., Tsai, C.-R., Chen, L.-H., Lee, H.-F., Mak, B. S.-C., Yang, S.-H., Wang, T.-Y., Shu, S.-G., Chen, C.-H. Maple syrup urine disease in the Austronesian aboriginal tribe Paiwan of Taiwan: a novel DBT (E2) gene 4.7 kb founder deletion caused by a nonhomologous recombination between LINE-1 and Alu and the carrier-frequency determination. Europ. J. Hum. Genet. 11: 931-936, 2003. 3. Chuang, D. T., Fisher, C. W., Lau, K. S., Griffin, T. A., Wynn, R. M., Cox, R. P. Maple syrup urine disease: domain structure, mutations and exon skipping in the dihydrolipoyl transacylase (E2) component of the branched-chain alpha-keto acid dehydrogenase complex. Molec. Biol. Med. 8: 49-63, 1991. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- DBT (Dihydrolipoamide Branched Chain Transacylase E2 (DBT))
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别名
- DBT
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物质类
- Viral Protein
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背景
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Synonyms: DBT, BCATE2, Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial, EC 2.3.1.168, 52 kDa mitochondrial autoantigen of primary biliary cirrhosis, Branched chain 2-oxo-acid dehydrogenase complex component E2, BCOADC-E2, Branched-chain alpha-keto acid dehydrogenase complex component E2, BCKAD-E2, BCKADE2, Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, Dihydrolipoamide branched chain transacylase, Dihydrolipoyllysine-residue, 2-methylpropanoyltransferase
Background: The branched-chain alpha-keto acid dehydrogenase complex (BCKD) is an inner-mitochondrial enzyme complex involved in the breakdown of the branched-chain amino acids isoleucine, leucine, and valine. The BCKD complex is thought to be composed of a core of 24 transacylase (E2) subunits, and associated decarboxylase (E1), dehydrogenase (E3), and regulatory subunits. This gene encodes the transacylase (E2) subunit. Mutations in this gene result in maple syrup urine disease, type 2. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.
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分子量
- 53 kDa
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基因ID
- 1629
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UniProt
- P11182
抗原
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