SMOX 抗体 (AA 45-454)
Quick Overview for SMOX 抗体 (AA 45-454) (ABIN7601776)
抗原
See all SMOX 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 45-454
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原理
- Anti-SMOX Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-SMOX Antibody Picoband® (ABIN7601776). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human SMOX recombinant protein (Position: E45-Q454).
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亚型
- IgG
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anti-Spermine Oxidase (SMOX) (Internal Region) antibody
SMOX 适用: 人, 小鼠, 大鼠 WB, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Spermine Oxidase (SMOX) (AA 1-200) antibodySMOX 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Spermine Oxidase (SMOX) (AA 1-165) antibodySMOX 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Spermine Oxidase (SMOX) (AA 268-353) antibodySMOX 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Spermine Oxidase (SMOX) (AA 1-555) antibodySMOX 适用: 小鼠 ELISA, WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Hartz, P. A. Personal Communication. Baltimore, Md. 6/20/2014. 2. Murray-Stewart, T., Wang, Y., Devereux, W., Casero, R. A., Jr. Cloning and characterization of multiple human polyamine oxidase splice variants that code for isoenzymes with different biochemical characteristics. Biochem. J. 368: 673-677, 2002. 3. Pledgie, A., Huang, Y., Hacker, A., Zhang, Z., Woster, P. M., Davidson, N. E., Casero, R. A., Jr. Spermine oxidase SMO(PAOh1), not N(1)-acetylpolyamine oxidase PAO, is the primary source of cytotoxic H2O2 in polyamine analogue-treated human breast cancer cell lines. J. Biol. Chem. 280: 39843-39851, 2005. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- SMOX (Spermine Oxidase (SMOX))
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别名
- SMOX
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背景
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Synonyms: Spermine oxidase, Polyamine oxidase 1, PAO-1, PAOh1, Smox, Smo
Tissue Specificity: Widely expressed. Isoform 1 and isoform 2 are expressed at higher level in brain and skeletal muscle. Isoform 7 is found in brain and spleen, isoform 10 is widely expressed but found at lower level in heart, kidney, liver and lung.
Background: Spermine oxidase is an enzyme that in humans is encoded by the SMOX gene. Polyamines are ubiquitous polycationic alkylamines which include spermine, spermidine, putrescine, and agmatine. These molecules participate in a broad range of cellular functions which include cell cycle modulation, scavenging reactive oxygen species, and the control of gene expression. These molecules also play important roles in neurotransmission through their regulation of cell-surface receptor activity, involvement in intracellular signalling pathways, and their putative roles as neurotransmitters. This gene encodes an FAD-containing enzyme that catalyzes the oxidation of spermine to spermadine and secondarily produces hydrogen peroxide. Multiple transcript variants encoding different isoenzymes have been identified for this gene, some of which have failed to demonstrate significant oxidase activity on natural polyamine substrates. The characterized isoenzymes have distinctive biochemical characteristics and substrate specificities, suggesting the existence of additional levels of complexity in polyamine catabolism.
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分子量
- 69 kDa
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基因ID
- 54498
抗原
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