SPRED1 抗体 (AA 45-322)
Quick Overview for SPRED1 抗体 (AA 45-322) (ABIN7601769)
抗原
See all SPRED1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 45-322
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原理
- Anti-SPRED1 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-SPRED1 Antibody Picoband® (ABIN7601769). Tested in WB, ICC/IF, Flow Cytometry, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human SPRED1 recombinant protein (Position: F45-K322). Human SPRED1 shares 89.9% amino acid (aa) sequence identity with mouse SPRED1.
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亚型
- IgG
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anti-Sprouty-Related, EVH1 Domain Containing 1 (SPRED1) (AA 123-311) antibody
SPRED1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Sprouty-Related, EVH1 Domain Containing 1 (SPRED1) antibodySPRED1 适用: 人 WB, IHC, IHC (p), FACS 宿主: 兔 Polyclonal unconjugated
anti-Sprouty-Related, EVH1 Domain Containing 1 (SPRED1) (AA 1-444) antibodySPRED1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Sprouty-Related, EVH1 Domain Containing 1 (SPRED1) (Middle Region 2) antibodySPRED1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Sprouty-Related, EVH1 Domain Containing 1 (SPRED1) (Middle Region 1) antibodySPRED1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Sprouty-Related, EVH1 Domain Containing 1 (SPRED1) (Center) antibodySPRED1 适用: 人, 小鼠, 大鼠 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-Sprouty-Related, EVH1 Domain Containing 1 (SPRED1) (N-Term) antibodySPRED1 适用: 人 WB, EIA 宿主: 兔 Polyclonal unconjugated
anti-Sprouty-Related, EVH1 Domain Containing 1 (SPRED1) (C-Term) antibodySPRED1 适用: 人 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL
1. Ablain, J., Xu, M., Rothschild, H., Jordan, R. C., Mito, J. K., Daniels, B. H., Bell, C. F., Joseph, N. M., Wu, H., Bastian, B. C., Zon, L. I., Yeh, I. Human tumor genomics and zebrafish modeling identify SPRED1 loss as a driver of mucosal melanoma. Science 362: 1055-1060, 2018. 2. Brems, H., Chmara, M., Sahbatou, M., Denayer, E., Taniguchi, K. Kato, R., Somers, R., Messiaen, L., De Schepper, S., Fryns, J.-P., Cools, J., Marynen, P., Thomas, G., Yoshimura, A., Legius, E. Germline loss-of-function mutations in SPRED1 cause a neurofibromatosis 1-like phenotype. (Letter) Nature Genet. 39: 1120-1126, 2007. 3. Brems, H., Pasmant, E., Van Minkelen, R., Wimmer, K., Upadhyaya, M., Legius, E., Messiaen, L. Review and update of SPRED1 mutations causing Legius syndrome. Hum. Mutat. 33: 1538-1546, 2012. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- SPRED1 (Sprouty-Related, EVH1 Domain Containing 1 (SPRED1))
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别名
- SPRED1
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背景
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Synonyms: SPRED1, Sprouty-related, EVH1 domain-containing protein 1, Spred-1, hSpred1
Background: The protein encoded by this gene is a member of the Sprouty family of proteins and is phosphorylated by tyrosine kinase in response to several growth factors. The encoded protein can act as a homodimer or as a heterodimer with SPRED2 to regulate activation of the MAP kinase cascade. Defects in this gene are a cause of neurofibromatosis type 1-like syndrome (NFLS).
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分子量
- 50 kDa
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基因ID
- 161742
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UniProt
- Q7Z699
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途径
- Positive Regulation of Response to DNA Damage Stimulus
抗原
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