POLA1 抗体 (AA 45-1429)
Quick Overview for POLA1 抗体 (AA 45-1429) (ABIN7601759)
抗原
See all POLA1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 45-1429
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原理
- Anti-POLA1 Antibody Picoband®
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交叉反应 (详细)
- No cross-reactivity with other proteins.
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产品特性
- Anti-POLA1 Antibody Picoband® (ABIN7601759). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human POLA1 recombinant protein (Position: E45-D1429).
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亚型
- IgG
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应用备注
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Western blot, 0.1-0.25 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Adler, D. A., Tseng, B. Y., Wang, T. S.-F., Disteche, C. M. Physical mapping of the genes for three components of the mouse DNA replication complex: polymerase alpha to the X chromosome, primase p49 subunit to chromosome 10, and primase p58 subunit to chromosome 1. Genomics 9: 642-646, 1991. 2. Hanaoka, F., Tandai, M., Miyazawa, H., Murakami, Y., Hori, T., Yamada, M. Assignment of human DNA polymerase alpha gene (POLA) to the X chromosome. (Abstract) Cytogenet. Cell Genet. 40: 647 only, 1985. 3. Miyazawa, H., Tandai, M., Hanaoka, F., Yamada, M., Hori, T., Shimizu, K., Sekiguchi, M. Identification of a DNA segment containing the human DNA polymerase alpha gene. Biochem. Biophys. Res. Commun. 139: 637-643, 1986. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- POLA1 (DNA Polymerase alpha (POLA1))
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别名
- POLA1
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背景
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Synonyms: Tumor necrosis factor ligand superfamily member 18, Activation-inducible TNF-related ligand, AITRL, Glucocorticoid-induced TNF-related ligand, hGITRL, TNFSF18, AITRL, GITRL, TL6, UNQ149/PRO175,
Tissue Specificity: Expressed at high levels in the small intestine, ovary, testis, kidney and endothelial cells.
Background: DNA polymerase alpha catalytic subunit is an enzyme that in humans is encoded by the POLA1 gene. This gene encodes the catalytic subunit of DNA polymerase, which together with a regulatory and two primase subunits, forms the DNA polymerase alpha complex. The catalytic subunit plays an essential role in the initiation of DNA replication.
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分子量
- 180 kDa
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基因ID
- 5422
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UniProt
- P09884
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途径
- SARS-CoV-2 Protein Interactome
抗原
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