ChT 抗体 (AA 446-580)
Quick Overview for ChT 抗体 (AA 446-580) (ABIN7601754)
抗原
See all ChT 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 446-580
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原理
- Anti-SLC5A7 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-SLC5A7 Antibody Picoband® (ABIN7601754). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human SLC5A7 recombinant protein (Position: R446-Q580).
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亚型
- IgG
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anti-High Affinity Choline Transporter (ChT) (AA 421-580) antibody
ChT 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-High Affinity Choline Transporter (ChT) (AA 501-580) antibodyChT 适用: 小鼠, 人, 大鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal 62-2E8 unconjugated
anti-High Affinity Choline Transporter (ChT) (AA 299-311), (Extracellular) antibodyChT 适用: 小鼠 WB, IHC, IF, IC, LCI 宿主: 兔 Polyclonal unconjugated
anti-High Affinity Choline Transporter (ChT) (AA 468-517) antibodyChT 适用: 小鼠, 人, 大鼠, 小鸡, Cow, 豚鼠, 兔, 马, Hamster, Pig, Bat WB 宿主: 兔 Polyclonal unconjugated
anti-High Affinity Choline Transporter (ChT) antibody (PE)ChT 适用: 小鸡 FACS 宿主: 小鼠 Monoclonal CT1 PE
anti-High Affinity Choline Transporter (ChT) antibodyChT 适用: 小鸡 FACS 宿主: 小鼠 Monoclonal CT1 unconjugated
anti-High Affinity Choline Transporter (ChT) antibody (Biotin)ChT 适用: 小鸡 FACS 宿主: 小鼠 Monoclonal CT1 Biotin
anti-High Affinity Choline Transporter (ChT) antibody (FITC)ChT 适用: 小鸡 FACS 宿主: 小鼠 Monoclonal CT1 FITC
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应用备注
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Western blot, 0.25-0.5 μg/mL/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/mL/1x10^6 cells, Human
ELISA, 0.1-0.5 μg/mL/mL, Human
1. Apparsundaram, S., Ferguson, S. M., George, A. L., Jr., Blakely, R. D. Molecular cloning of a human, hemicholinium-3-sensitive choline transporter. Biochem. Biophys. Res. Commun. 276: 862-867, 2000. 2. Barwick, K. E. S., Wright, J., Al-Turki, S., McEntagart, M. M., Nair, A., Chioza, B., Al-Memar, A., Modarres, H., Reilly, M. M., Dick, K. J., Ruggiero, A. M., Blakely, R. D., Hurles, M. E., Crosby, A. H. Defective presynaptic choline transport underlies hereditary motor neuropathy. Am. J. Hum. Genet. 91: 1103-1107, 2012. 3. Bauche, S., O'Regan, S., Azuma, Y., Laffargue, F., McMacken, G., Sternberg, D., Brochier, G., Buon, C., Bouzidi, N., Topf, A., Lacene, E., Remerand, G., and 30 others. Impaired presynaptic high-affinity choline transporter causes a congenital myasthenic syndrome with episodic apnea. Am. J. Hum. Genet. 99: 753-761, 2016. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- ChT (High Affinity Choline Transporter (ChT))
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别名
- SLC5A7
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背景
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Synonyms: Transcription factor MafA, Pancreatic beta-cell-specific transcriptional activator, RIPE3b1 factor, V-maf musculoaponeurotic fibrosarcoma oncogene homolog A, MAFA
Tissue Specificity: Preferentially expressed in regulatory T-cells (Tregs).
Background: The high-affinity choline transporter (ChT) also known as solute carrier family 5 member 7 is a protein in humans that is encoded by the SLC5A7 gene. This gene encodes a sodium ion- and chloride ion-dependent high-affinity transporter that mediates choline uptake for acetylcholine synthesis in cholinergic neurons. The protein transports choline from the extracellular space into presynaptic terminals for synthesis into acetylcholine. Increased choline uptake results from increased density of this protein in synaptosomal plasma membranes in response to depolarization of cholinergic terminals. Dysfunction of cholinergic signaling has been implicated in various disorders including depression, attention-deficit disorder, and schizophrenia. An allelic variant of this gene is associated with autosomal dominant distal hereditary motor neuronopathy type VIIA. Alternative splicing results in multiple transcript variants.
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分子量
- 80 kDa
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基因ID
- 60482
抗原
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