NNT 抗体 (AA 44-350)
Quick Overview for NNT 抗体 (AA 44-350) (ABIN7601742)
抗原
See all NNT 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 44-350
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原理
- Anti-NNT Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-NNT Antibody Picoband® (ABIN7601742). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat, Pig. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human NNT recombinant protein (Position: C44-A350).
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亚型
- IgG
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anti-Nicotinamide Nucleotide Transhydrogenase (NNT) (AA 1-207) antibody
NNT 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1D6 unconjugated
anti-Nicotinamide Nucleotide Transhydrogenase (NNT) (C-Term) antibodyKO Validated NNT 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Nicotinamide Nucleotide Transhydrogenase (NNT) (N-Term) antibodyNNT 适用: 人, 小鼠, 大鼠, 犬, Cow, 斑马鱼, 豚鼠, 马, 兔 WB 宿主: 兔 Polyclonal unconjugated
anti-Nicotinamide Nucleotide Transhydrogenase (NNT) (AA 981-1086) antibodyNNT 适用: 人, 小鼠, 犬 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Nicotinamide Nucleotide Transhydrogenase (NNT) (C-Term) antibodyNNT 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nicotinamide Nucleotide Transhydrogenase (NNT) (AA 1-207) antibodyNNT 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Nicotinamide Nucleotide Transhydrogenase (NNT) (AA 252-301) antibodyNNT 适用: 人, 小鼠, 大鼠, Cow, Pig, 豚鼠, 马, 兔, 猴, Bat, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
anti-Nicotinamide Nucleotide Transhydrogenase (NNT) (AA 881-1086) antibodyNNT 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Nicotinamide Nucleotide Transhydrogenase (NNT) (N-Term) antibodyNNT 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.25 μg/mL, Human, Mouse, Monkey, Rat, Pig
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Hasselmann, C., Deladoey, J., Vuissoz, J.-M., Patry, L., Alirezaie, N., Schwartzentruber, J., FORGE Canada Consortium, Deal, C. L., Van Vliet, G., Majewski, J., Samuels, M. E. Expanding the phenotypic spectrum of nicotinamide nucleotide transhydrogenase (NNT) mutations and using whole exome sequencing to discover potential disease modifiers. J. Genomes Exomes 2: 19-30, 2013. 2. Meimaridou, E., Kowalczyk, J., Guasti, L., Hughes, C. R., Wagner, F., Frommolt, P., Nurnberg, P., Mann, N. P., Banerjee, R., Saka, H. N., Chapple, J. P., King, P. J., Clark, A. J. L., Metherell, L. A. Mutations in NNT encoding nicotinamide nucleotide transhydrogenase cause familial glucocorticoid deficiency. Nature Genet. 44: 740-742, 2012. 3. Roucher-Boulez, F., Mallet-Motak, D., Samara-Boustani, D., Jilani, H., Ladjouze, A., Souchon, P.-F., Simon, D., Nivot, S., Heinrichs, C., Ronze, M., Bertagna, X., Groisne, L., Leheup, B., Naud-Saudreau, C., Blondin, G., Lefevre, C., Lemarchand, L., Morel, Y. NNT mutations: a cause of primary adrenal insufficiency, oxidative stress and extra-adrenal defects. Europ. J. Endocr. 175: 73-84, 2016. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- NNT (Nicotinamide Nucleotide Transhydrogenase (NNT))
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别名
- NNT
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背景
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Synonyms: Glucokinase, Hexokinase type IV, HK IV, Hexokinase-4, HK4, Hexokinase-D, GCK
Tissue Specificity: Isoform 1 is expressed in pancreas. Isoform 2 and isoform 3 is expressed in liver.
Background: This gene encodes an integral protein of the inner mitochondrial membrane. The enzyme couples hydride transfer between NAD (H) and NADP (+) to proton translocation across the inner mitochondrial membrane. Under most physiological conditions, the enzyme uses energy from the mitochondrial proton gradient to produce high concentrations of NADPH. The resulting NADPH is used for biosynthesis and in free radical detoxification.
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分子量
- 114 kDa
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基因ID
- 23530
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UniProt
- Q13423
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途径
- Proton Transport
抗原
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