NARF 抗体 (AA 41-353)
Quick Overview for NARF 抗体 (AA 41-353) (ABIN7601665)
抗原
See all NARF 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 41-353
-
原理
- Anti-NARF Antibody Picoband®
-
交叉反应 （详细）
- No cross-reactivity with other proteins.
-
产品特性
- Anti-NARF Antibody Picoband® (ABIN7601665). Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
-
纯化方法
- Immunogen affinity purified.
-
免疫原
- E.coli-derived human NARF recombinant protein (Position: H41-Q353).
-
亚型
- IgG
-
-
anti-Nuclear Prelamin A Recognition Factor (NARF) antibody
NARF 适用: 小鼠, 大鼠 IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Prelamin A Recognition Factor (NARF) (AA 10-41) antibodyNARF 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Prelamin A Recognition Factor (NARF) (C-Term) antibodyNARF 适用: 人, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Prelamin A Recognition Factor (NARF) antibodyNARF 适用: 人, 小鼠, 大鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Prelamin A Recognition Factor (NARF) (AA 1-100) antibodyNARF 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 7D9 unconjugated
anti-Nuclear Prelamin A Recognition Factor (NARF) (AA 323-372) antibodyNARF 适用: 人, 马, 兔, Cow, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Prelamin A Recognition Factor (NARF) (AA 395-444) antibodyNARF 适用: 人, 马, 兔, Pig, 猴 WB 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg /1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Barton, R. M., Worman, H. J. Prenylated prelamin A interacts with Narf, a novel nuclear protein. J. Biol. Chem. 274: 30008-30018, 1999. -
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
-
浓度
- 500 μg/mL
-
缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
-
储存条件
- 4 °C,-20 °C
-
储存方法
-
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
-
-
- NARF (Nuclear Prelamin A Recognition Factor (NARF))
-
别名
- NARF
-
背景
-
Synonyms: E3 ubiquitin-protein ligase RNF169, RING finger protein 169, RING-type E3 ubiquitin transferase RNF169, RNF169, KIAA1991
Tissue Specificity: Expressed in immature but not mature T-cells. Also found in CD34+ cells from peripheral blood, CD34+ precursors from umbilical cord blood and adult bone marrow.
Background: Several proteins have been found to be prenylated and methylated at their carboxyl-terminal ends. Prenylation was initially believed to be important only for membrane attachment. However, another role for prenylation appears to be its importance in protein-protein interactions. The only nuclear proteins known to be prenylated in mammalian cells are prelamin A- and B-type lamins. Prelamin A is farnesylated and carboxymethylated on the cysteine residue of a carboxyl-terminal CaaX motif. This post-translationally modified cysteine residue is removed from prelamin A when it is endoproteolytically processed into mature lamin A. The protein encoded by this gene binds to the prenylated prelamin A carboxyl-terminal tail domain. It may be a component of a prelamin A endoprotease complex. The encoded protein is located in the nucleus, where it partially colocalizes with the nuclear lamina. It shares limited sequence similarity with iron-only bacterial hydrogenases. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene, including one with a novel exon that is generated by RNA editing.
-
分子量
- 52 kDa
-
基因ID
- 26502
抗原
-
