GIT2 抗体 (AA 400-556)
Quick Overview for GIT2 抗体 (AA 400-556) (ABIN7601655)
抗原
See all GIT2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 400-556
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原理
- Anti-GIT2 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-GIT2 Antibody Picoband® (ABIN7601655). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human GIT2 recombinant protein (Position: D400-S556).
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亚型
- IgG
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anti-G Protein-Coupled Receptor Kinase Interactor 2 (GIT2) (AA 1-471) antibody
GIT2 适用: 人 WB, ELISA, IP, IF 宿主: 小鼠 Monoclonal M1 unconjugated
anti-G Protein-Coupled Receptor Kinase Interactor 2 (GIT2) (AA 1-471) antibodyGIT2 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 3B5-B9 unconjugated
anti-G Protein-Coupled Receptor Kinase Interactor 2 (GIT2) (AA 1-471) antibodyGIT2 适用: 人 WB, FACS 宿主: 小鼠 Polyclonal unconjugated
anti-G Protein-Coupled Receptor Kinase Interactor 2 (GIT2) (Internal Region) antibodyGIT2 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor Kinase Interactor 2 (GIT2) (AA 1-603) antibodyGIT2 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor Kinase Interactor 2 (GIT2) (Internal Region) antibodyGIT2 适用: 人, 大鼠, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor Kinase Interactor 2 (GIT2) (AA 1-471) antibodyGIT2 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor Kinase Interactor 2 (GIT2) (AA 401-498) antibodyGIT2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1B2 unconjugated
anti-G Protein-Coupled Receptor Kinase Interactor 2 (GIT2) (AA 714-749), (C-Term) antibodyGIT2 适用: 人 WB 宿主: 兔 Polyclonal RB48356 unconjugated
anti-G Protein-Coupled Receptor Kinase Interactor 2 (GIT2) (AA 1-312) antibodyGIT2 适用: 人 WB, IHC, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.25 μg/mL, Human, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Bagrodia, S., Bailey, D., Lenard, Z., Hart, M., Guan, J. L., Premont, R. T., Taylor, S. J., Cerione, R. A. A tyrosine-phosphorylated protein that binds to an important regulatory region on the Cool family of p21-activated kinase-binding proteins. J. Biol. Chem. 274: 22393-22400, 1999. 2. Mazaki, Y., Hashimoto, S., Okawa, K., Tsubouchi, A., Nakamura, K., Yagi, R., Yano, H., Kondo, A., Iwamatsu, A., Mizoguchi, A., Sabe, H. An ADP-ribosylation factor GTPase-activating protein Git2-short/KIAA0148 is involved in subcellular localization of paxillin and actin cytoskeletal organization. Molec. Biol. Cell 12: 645-662, 2001. 3. Nagase, T., Seki, N., Tanaka, A., Ishikawa, K., Nomura, N. Prediction of the coding sequences of unidentified human genes. IV. The coding sequences of 40 new genes (KIAA0121-KIAA0160) deduced by analysis of cDNA clones from human cell line KG-1. DNA Res. 2: 167-174, 1995. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- GIT2 (G Protein-Coupled Receptor Kinase Interactor 2 (GIT2))
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别名
- GIT2
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背景
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Synonyms: Interleukin-25, IL-25, Interleukin-17E, IL-17E, IL25, IL17E, UNQ3120/PRO10272
Tissue Specificity: Expressed at low levels in several tissues, including brain, kidney, lung, prostate, testis, spinal cord, adrenal gland, and trachea.
Background: ARF GTPase-activating protein GIT2 is an enzyme that in humans is encoded by the GIT2 gene. This gene encodes a member of the GIT protein family, which interact with G protein-coupled receptor kinases and possess ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) activity. GIT proteins traffic between cytoplasmic complexes, focal adhesions, and the cell periphery, and interact with Pak interacting exchange factor beta (PIX) to form large oligomeric complexes that transiently recruit other proteins. GIT proteins regulate cytoskeletal dynamics and participate in receptor internalization and membrane trafficking. This gene has been shown to repress lamellipodial extension and focal adhesion turnover, and is thought to regulate cell motility. This gene undergoes extensive alternative splicing to generate multiple isoforms, but the full-length nature of some of these variants has not been determined. The various isoforms have functional differences, with respect to ARF GAP activity and to G protein-coupled receptor kinase 2 binding.
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分子量
- 85 kDa
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基因ID
- 9815
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UniProt
- Q14161
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途径
- Regulation of G-Protein Coupled Receptor Protein Signaling
抗原
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