ARHGEF12 抗体 (AA 4-254)
Quick Overview for ARHGEF12 抗体 (AA 4-254) (ABIN7601615)
抗原
See all ARHGEF12 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 4-254
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原理
- Anti-LARG/ARHGEF12 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-LARG/ARHGEF12 Antibody Picoband® (ABIN7601615). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human LARG/ARHGEF12 recombinant protein (Position: T4-S254).
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亚型
- IgG
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anti-rho Guanine Nucleotide Exchange Factor (GEF) 12 (ARHGEF12) (AA 1302-1501) antibody
ARHGEF12 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-rho Guanine Nucleotide Exchange Factor (GEF) 12 (ARHGEF12) antibodyARHGEF12 适用: 人, 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-rho Guanine Nucleotide Exchange Factor (GEF) 12 (ARHGEF12) (Internal Region) antibodyARHGEF12 适用: 人 ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-rho Guanine Nucleotide Exchange Factor (GEF) 12 (ARHGEF12) (AA 817-916) antibodyARHGEF12 适用: 人 ELISA, WB 宿主: 小鼠 Polyclonal unconjugated
anti-rho Guanine Nucleotide Exchange Factor (GEF) 12 (ARHGEF12) (Internal Region) antibodyARHGEF12 适用: 人, 小鼠, 大鼠 ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-rho Guanine Nucleotide Exchange Factor (GEF) 12 (ARHGEF12) (Internal Region) antibodyARHGEF12 适用: 人, 小鼠, 大鼠 ELISA, WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Goulimari, P., Knieling, H., Engel, U., Grosse, R. LARG and mDia1 link G-alpha-12/13 to cell polarity and microtubule dynamics. Molec. Biol. Cell 19: 30-40, 2008. 2. Kourlas, P. J., Strout, M. P., Becknell, B., Veronese, M. L., Croce, C. M., Theil, K. S., Krahe, R., Ruutu, T., Knuutila, S., Bloomfield, C. D., Caligiuri, M. A. Identification of a gene at 11q23 encoding a guanine nucleotide exchange factor: evidence for its fusion with MLL in acute myeloid leukemia. Proc. Nat. Acad. Sci. 97: 2145-2150, 2000. 3. Martz, M. K., Grabocka, E., Beeharry, N., Yen, T. J., Wedegaertner, P. B. Leukemia-associated RhoGEF (LARG) is a novel RhoGEF in cytokinesis and required for the proper completion of abscission. Molec. Biol. Cell 24: 2785-2794, 2013. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- ARHGEF12 (rho Guanine Nucleotide Exchange Factor (GEF) 12 (ARHGEF12))
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别名
- ARHGEF12
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背景
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Synonyms: PR domain zinc finger protein 5, PR domain-containing protein 5, PRDM5, PFM2
Tissue Specificity: Widely expressed with highest levels in colon and ovary. Tends to be silenced in breast, colorectal, gastric and liver cancer tissues.
Background: Rho guanine nucleotide exchange factor 12 is a protein that in humans is encoded by the ARHGEF12 gene. Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli working through G protein-coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein has been observed to form a myeloid/lymphoid fusion partner in acute myeloid leukemia. Three transcript variants encoding different isoforms have been found for this gene.
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分子量
- 220 kDa
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基因ID
- 23365
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途径
- Neurotrophin Signaling Pathway, Regulation of G-Protein Coupled Receptor Protein Signaling
抗原
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