DHX9 抗体 (AA 396-759)
Quick Overview for DHX9 抗体 (AA 396-759) (ABIN7601604)
抗原
See all DHX9 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 396-759
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原理
- Anti-RNA Helicase A/DHX9 Antibody Picoband®
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交叉反应 (详细)
- No cross-reactivity with other proteins.
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产品特性
- Rabbit IgG polyclonal antibody for RNA Helicase A/DHX9 detection. Tested with WB, IHC, ICC/IF, IF, Flow Cytometry, ELISA in Human,Mouse,Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human RNA Helicase A/DHX9 recombinant protein (Position: E396-E759).
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亚型
- IgG
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应用备注
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Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Abdelhaleem, M. M., Hameed, S., Klassen, D., Greenberg, A. H. Leukophysin: an RNA helicase A-related molecule identified in cytotoxic T cell granules and vesicles. J. Immun. 156: 2026-2035, 1996. 2. Aktas, T., Avsar Ilik, I., Maticzka, D., Bhardwaj, V., Pessoa Rodrigues, C., Mittler, G., Manke, T., Backofen, R., Akhtar, A. DHX9 suppresses RNA processing defects originating from the Alu invasion of the human genome. Nature 544: 115-119, 2017. 3. Lee, C. G., Hurwitz, J. A new RNA helicase isolated from HeLa cells that catalytically translocates in the 3-prime to 5-prime ion. J. Biol. Chem. 267: 4398-4407, 1992. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- DHX9 (ATP-Dependent RNA Helicase A (DHX9))
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别名
- DHX9
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背景
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Synonyms: ATP-dependent RNA helicase A, DEAH box protein 9, DExH-box helicase 9, Leukophysin, LKP, Nuclear DNA helicase II, NDH II, RNA helicase A, DHX9, DDX9
Tissue Specificity: Macrophages, peripheral blood leukocytes, lung, spleen and liver.
Background: ATP-dependent RNA helicase A (RHA, also known as DHX9, LKP, and NDHI) is an enzyme that in humans is encoded by the DHX9 gene. It is mapped to 1q25.3. This gene encodes a member of the DEAH-containing family of RNA helicases. The encoded protein is an enzyme that catalyzes the ATP-dependent unwinding of double-stranded RNA and DNA-RNA complexes. This protein localizes to both the nucleus and the cytoplasm and functions as a transcriptional regulator. This protein may also be involved in the expression and nuclear export of retroviral RNAs. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 11 and 13.
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分子量
- 141 kDa
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基因ID
- 1660
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UniProt
- Q08211
抗原
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