Glutaminase 抗体 (AA 396-654)
Quick Overview for Glutaminase 抗体 (AA 396-654) (ABIN7601603)
抗原
See all Glutaminase (GLS) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 396-654
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原理
- Anti-Glutaminase/GLS Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-Glutaminase/GLS Antibody Picoband® (ABIN7601603). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human Glutaminase/GLS recombinant protein (Position: K396-N654).
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亚型
- IgG
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anti-Glutaminase (GLS) (AA 31-253) antibody
GLS 适用: 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Glutaminase (GLS) (AA 516-545), (C-Term) antibodyGLS 适用: 人, 小鼠, 大鼠 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB22875 unconjugated
anti-Glutaminase (GLS) (AA 580-669) antibodyGLS 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 5C4 unconjugated
anti-Glutaminase (GLS) (AA 610-669) antibodyGLS 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Glutaminase (GLS) (AA 221-533) antibodyGLS 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glutaminase (GLS) antibodyMicroarray verified GLS 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB601157 unconjugated
anti-Glutaminase (GLS) (C-Term) antibodyGLS 适用: 人, 小鼠, 大鼠, 兔, 犬, 豚鼠, 马 WB 宿主: 兔 Polyclonal unconjugated
anti-Glutaminase (GLS) (AA 616-669) antibodyGLS 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Glutaminase (GLS) (AA 580-669) antibodyGLS 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Glutaminase (GLS) (AA 144-177) antibodyGLS 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal RB53876 unconjugated
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应用备注
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Western blot, 0.1-0.25 μg/mL, Human, Monkey, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat
Immunocytochemistry/Immunofluorescence, 4 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Lynch, D. S., Chelban, V., Vandrovcova, J., Pittman, A., Wood, N. W., Houlden, H. GLS loss of function causes autosomal recessive spastic ataxia and optic atrophy. Ann. Clin. Transl. Neurol. 5: 216-221, 2018. 2. Modi, W. S., Pollock, D. D., Mock, B. A., Banner, C., Renauld, J.-C., Van Snick, J. Regional localization of the human glutaminase (GLS) and interleukin-9 (IL9) genes by in situ hybridization. Cytogenet. Cell Genet. 57: 114-116, 1991. 3. Rumping, L., Tessadori, F., Pouwels, P. J. W., Vringer, E., Wijnen, J. P., Bhogal, A. A., Savelberg, S. M. C., Duran, K. J., Bakkers, M. J. G., Ramos, R. J. J., Schellekens, P. A. W., Kroes, H. Y., and 16 others. GLS hyperactivity causes glutamate excess, infantile cataract and profound developmental delay. Hum. Molec. Genet. 28: 96-104, 2019. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- Glutaminase (GLS)
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别名
- GLS
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背景
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Synonyms: [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial, Pyruvate dehydrogenase kinase isoform 1, PDH kinase 1, PDK1, PDHK1
Tissue Specificity: Expressed predominantly in the heart. Detected at lower levels in liver, skeletal muscle and pancreas.
Background: This gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants.
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分子量
- 65-73 kDa
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基因ID
- 2744
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UniProt
- O94925
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途径
- Feeding Behaviour, Dicarboxylic Acid Transport, Warburg Effect
抗原
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