PLD6 抗体 (AA 39-238)
Quick Overview for PLD6 抗体 (AA 39-238) (ABIN7601569)
抗原
See all PLD6 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 39-238
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原理
- Anti-PLD6 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-PLD6 Antibody Picoband® (ABIN7601569). Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human PLD6 recombinant protein (Position: R39-L238).
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亚型
- IgG
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anti-Phospholipase D Family, Member 6 (PLD6) (AA 1-252) antibody
PLD6 适用: 人 ELISA, WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Phospholipase D Family, Member 6 (PLD6) (AA 125-154) antibodyPLD6 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal RB28958 unconjugated
anti-Phospholipase D Family, Member 6 (PLD6) (AA 125-154) antibodyPLD6 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Choi, S.-Y., Huang, P., Jenkins, G. M., Chan, D. C., Schiller, J., Frohman, M. A. A common lipid links Mfn-mediated mitochondrial fusion and SNARE-regulated exocytosis. Nature Cell Biol. 8: 1255-1262, 2006. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 11/29/2012. 3. Huang, H., Gao, Q., Peng, X., Choi, S.-Y., Sarma, K., Ren, H., Morris, A. J., Frohman, M. A. piRNA-associated germline nuage formation and spermatogenesis require MitoPLD profusogenic mitochondrial-surface lipid signaling. Dev. Cell 20: 376-387, 2011. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- PLD6 (Phospholipase D Family, Member 6 (PLD6))
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别名
- PLD6
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背景
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Synonyms: Interleukin-17B, IL-17B, Cytokine CX1, Cytokine-like protein ZCYTO7, Neuronal interleukin-17-related factor, Il17b, Nirf, Zcyto7
Tissue Specificity: Expressed in adult pancreas, small intestine, stomach, spinal cord and testis. Less pronounced expression in prostate, colon mucosal lining, and ovary.
Background: PLD6 (phospholipase D family, member 6) is a protein-coding gene. Among its related super-pathways are choline biosynthesis III and Glycerophospholipid biosynthesis. GO annotations related to this gene include cardiolipin hydrolase activity and protein homodimerization activity. Regulates mitochondrial shape through facilitating mitochondrial fusion. During spermatogenesis, plays a critical role in PIWI-interacting RNA (piRNA) biogenesis (By similarity). piRNAs provide essential protection against the activity of mobile genetic elements. piRNA-mediated transposon silencing is thus critical for maintaining genome stability, in particular in germline cells when transposons are mobilized as a consequence of wide-spread genomic demethylation. Has been shown to be a backbone-non-specific, single strand-specific nuclease, cleaving either RNA or DNA substrates with similar affinity (By similarity). Produces 5' phosphate and 3' hydroxyl termini, suggesting it could ly participate in the processing of primary piRNA transcripts (By similarity). Has been proposed to act as a cardiolipin hydrolase to generate phosphatidic acid at mitochondrial surface. Although it cannot be excluded that it can act as a phospholipase in some circumstances, it should be noted that cardiolipin hydrolase activity is either undetectable in vitro, or very low (PubMed:21397848). In addition, cardiolipin is almost exclusively found on the inner mitochondrial membrane, while PLD6 localizes to the outer mitochondrial membrane, facing the cytosol.
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分子量
- 28 kDa
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基因ID
- 201164
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UniProt
- Q8N2A8
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途径
- Ribonucleoprotein Complex Subunit Organization
抗原
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