RhoF 抗体 (AA 39-204)
Quick Overview for RhoF 抗体 (AA 39-204) (ABIN7601568)
抗原
See all RhoF (RHOF) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
 - AA 39-204
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原理
 - Anti-RHOF Antibody Picoband®
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交叉反应 (详细)
 - No cross-reactivity with other proteins.
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产品特性
 - Anti-RHOF Antibody Picoband® (ABIN7601568). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
 - Immunogen affinity purified.
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免疫原
 - E.coli-derived human RHOF recombinant protein (Position: Y39-K204).
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亚型
 - IgG
 
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应用备注
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                        Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Ellis, S., Mellor, H. The novel Rho-family GTPase Rif regulates coordinated actin-based membrane rearrangements. Curr. Biol. 10: 1387-1390, 2000. 2. Fan, L., Pellegrin, S., Scott, A., Mellor, H. The small GTPase Rif is an alternative trigger for the formation of actin stress fibers in epithelial cells. J. Cell Sci. 123: 1247-1252, 2010. 3. Fan, L., Yan, H., Pellegrin, S., Morigen, Mellor, H. The Rif GTPase regulates cytoskeletal signaling from plexinA4 to promote neurite retraction. Neurosci. Lett. 590: 178-183, 2015. - 
                                            
限制
 - 仅限研究用
 
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状态
 - Lyophilized
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溶解方式
 - Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
 - 500 μg/mL
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缓冲液
 - Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
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储存条件
 - 4 °C,-20 °C
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储存方法
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                        Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. 
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. 
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- RhoF (RHOF) (Rho-related GTP-binding protein RhoF (RHOF))
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别名
 - RHOF
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背景
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Synonyms: Pannexin-2, PANX2
Tissue Specificity: Expressed in fetal and adult brain. Also detected in fetal liver and skeletal muscle, but not in their adult counterparts.
Background: Rho, the Ras-related small GTPase, is responsible for the regulation of actin-based cytoskeletal structures including stress fibers, focal adhesions and the contractile ring apparatus. Rho proteins function as molecular switches that are able to turn cytokinesis on and off. Although little is known about signaling downstream of Rho, a host of putative Rho effector proteins have been described. Rho F (ras homolog gene family, member F), also known as RIF or ARHF, is a 211 amino acid membrane protein that localizes to the cytoplasmic side of the plasma membrane. Belonging to the small GTPase superfamily and the Rho family, Rho F functions cooperatively with Cdc42 and Rac to generate additional cytoskeletal structures, such as increasing variation of actin-based morphology. Rho F exists as two alternatively spliced isoforms and is encoded by a gene located on human chromosome 12q24.31.
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分子量
 - 23 kDa
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基因ID
 - 54509
 
抗原
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