Symplekin 抗体 (AA 384-1092)
Quick Overview for Symplekin 抗体 (AA 384-1092) (ABIN7601558)
抗原
See all Symplekin (SYMPK) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 384-1092
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原理
- Anti-Symplekin/SYMPK Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-Symplekin/SYMPK Antibody Picoband® (ABIN7601558). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human Symplekin/SYMPK recombinant protein (Position: K384-N1092).
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亚型
- IgG
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anti-Symplekin (SYMPK) (AA 1-533) antibody
SYMPK 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 4C2 unconjugated
anti-Symplekin (SYMPK) (AA 1-347) antibodySYMPK 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Symplekin (SYMPK) (AA 1-533) antibodySYMPK 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Symplekin (SYMPK) (AA 1-533) antibodySYMPK 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Symplekin (SYMPK) (AA 180-229) antibodySYMPK 适用: 人, 兔, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Symplekin (SYMPK) (AA 310-530) antibodySYMPK 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Symplekin (SYMPK) (N-Term) antibodySYMPK 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Symplekin (SYMPK) (N-Term) antibodySYMPK 适用: 人, 小鼠, 大鼠, 兔, Cow, 犬, 豚鼠, 马 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Symplekin (SYMPK) (Middle Region) antibodySYMPK 适用: 人, 大鼠, Pig WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Alwazzan, M., Hamshere, M. G., Lennon, G. G., Brook, J. D. Six transcripts map within 200 kilobases of the myotonic dystrophy expanded repeat. Mammalian Genome 9: 485-487, 1998. 2. Keon, B. H., Schafer, S., Kuhn, C., Grund, C., Franke, W. W. Symplekin, a novel type of tight junction plaque protein. J. Cell Biol. 134: 1003-1018, 1996. 3. Kolev, N. G., Steitz, J. A. Symplekin and multiple other polyadenylation factors participate in 3-prime-end maturation of histone mRNAs. Genes Dev. 19: 2583-2592, 2005. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- Symplekin (SYMPK)
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别名
- SYMPK
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背景
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Synonyms: N-alpha-acetyltransferase 15, NatA auxiliary subunit,Gastric cancer antigen Ga19,N-terminal acetyltransferase,NMDA receptor-regulated protein 1,Protein tubedown-1,Tbdn100,NAA15,GA19, NARG1, NATH, TBDN100,
Tissue Specificity: Expressed at high levels in testis and in ocular endothelial cells. Also found in brain (corpus callosum), heart, colon, bone marrow and at lower levels in most adult tissues, including thyroid, liver, pancreas, mammary and salivary glands, lung, ovary, urogenital system and upper gastrointestinal tract. Overexpressed in gastric cancer, in papillary thyroid carcinomas and in a Burkitt lymphoma cell line (Daudi). Specifically suppressed in abnormal proliferating blood vessels in eyes of patients with proliferative diabetic retinopathy. .
Background: Symplekin is a protein that in humans is encoded by the SYMPK gene. This gene encodes a nuclear protein that functions in the regulation of polyadenylation and promotes gene expression. The protein forms a high-molecular weight complex with components of the polyadenylation machinery. It is thought to serve as a scaffold for recruiting regulatory factors to the polyadenylation complex. It also participates in 3'-end maturation of histone mRNAs, which do not undergo polyadenylation. The protein also localizes to the cytoplasmic plaques of tight junctions in some cell types.
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分子量
- 150 kDa
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基因ID
- 8189
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UniProt
- Q92797
抗原
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