PI4KB 抗体 (AA 375-671)
Quick Overview for PI4KB 抗体 (AA 375-671) (ABIN7601530)
抗原
See all PI4KB 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 375-671
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原理
- Anti-PI4KB Antibody Picoband®
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交叉反应 （详细）
- No cross reactivity with other proteins.
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产品特性
- Anti-PI4KB Antibody Picoband® (ABIN7601530). Tested in ELISA, IHC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human PI4KB recombinant protein (Position: D375-D671). Human PI4KB shares 100% and 99.7% amino acid (aa) sequence identity with mouse and rat PI4KB, respectively
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亚型
- IgG
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anti-Phosphatidylinositol 4-Kinase, Catalytic, beta (PI4KB) (AA 20-51), (N-Term) antibody
PI4KB 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB1676 unconjugated
anti-Phosphatidylinositol 4-Kinase, Catalytic, beta (PI4KB) (AA 560-801) antibodyPI4KB 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Phosphatidylinositol 4-Kinase, Catalytic, beta (PI4KB) (AA 731-828) antibodyPI4KB 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3B1 unconjugated
anti-Phosphatidylinositol 4-Kinase, Catalytic, beta (PI4KB) (AA 519-548) antibodyPI4KB 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB18563 unconjugated
anti-Phosphatidylinositol 4-Kinase, Catalytic, beta (PI4KB) (Middle Region) antibodyPI4KB 适用: 人 WB, IHC (p), FACS, EIA 宿主: 兔 Polyclonal unconjugated
anti-Phosphatidylinositol 4-Kinase, Catalytic, beta (PI4KB) (AA 755-804) antibodyPI4KB 适用: 人, 小鼠, 大鼠, 犬, 兔, 斑马鱼, Cow, 豚鼠, 马, 非洲爪蟾, Bat, Hamster, 猴, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Phosphatidylinositol 4-Kinase, Catalytic, beta (PI4KB) (AA 251-300) antibodyPI4KB 适用: 人, 小鼠, 大鼠, 犬, 兔, 斑马鱼, Cow, 豚鼠, 马, 非洲爪蟾, Bat, Hamster, 猴, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Phosphatidylinositol 4-Kinase, Catalytic, beta (PI4KB) (N-Term) antibodyPI4KB 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Phosphatidylinositol 4-Kinase, Catalytic, beta (PI4KB) (Middle Region) antibodyPI4KB 适用: 人, 小鼠, 大鼠, 犬, 兔, 斑马鱼, Cow, 豚鼠, 马 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Burke, J. E., Inglis, A. J., Perisic, O., Masson, G. R., McLaughlin, S. H., Rutaganira, F., Shokat, K. M., Williams, R. L. Structures of PI4KIII-beta complexes show simultaneous recruitment of Rab11 and its effectors. Science 344: 1035-1038, 2014. 2. Gromada, J., Bark, C., Smidt, K., Efanov, A. M., Janson, J., Mandic, S. A., Webb, D.-L., Zhang, W., Meister, B., Jeromin, A., Berggren, P.-O. Neuronal calcium sensor-1 potentiates glucose-dependent exocytosis in pancreatic beta cells through activation of phosphatidylinositol 4-kinase beta. Proc. Nat. Acad. Sci. 102: 10303-10308, 2005. 3. Jovic, M., Kean, M. J., Szentpetery, Z., Polevoy, G., Gingras, A.-C., Brill, J. A., Balla, T. Two phosphatidylinositol 4-kinases control lysosomal delivery of the Gaucher disease enzyme, beta-glucocerebrosidase. Molec. Biol. Cell 23: 1533-1545, 2012. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- PI4KB (Phosphatidylinositol 4-Kinase, Catalytic, beta (PI4KB))
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别名
- PI4KB
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背景
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Synonyms: 70 kDa ribosomal protein S6 kinase 1 antibody, KS6B1_HUMAN antibody, p70 alpha antibody, P70 beta 1 antibody, p70 ribosomal S6 kinase alpha antibody, p70 ribosomal S6 kinase beta 1 antibody, p70 S6 kinase alpha antibody, P70 S6 Kinase antibody, p70 S6 kinase alpha 1 antibody, p70 S6 kinase alpha 2 antibody, p70 S6K antibody, p70 S6K-alpha antibody, p70 S6KA antibody, p70(S6K) alpha antibody, p70(S6K)-alpha antibody, p70-alpha antibody, p70-S6K 1 antibody, p70-S6K antibody, P70S6K antibody, P70S6K1 antibody, p70S6Kb antibody, PS6K antibody, Ribosomal protein S6 kinase 70 kDa polypeptide 1 antibody, Ribosomal protein S6 kinase beta 1 antibody, Ribosomal protein S6 kinase beta-1 antibody, Ribosomal protein S6 kinase I antibody, RPS6KB1 antibody, S6K antibody, S6K-beta-1 antibody, S6K1 antibody, Serine/threonine kinase 14 alpha antibody, Serine/threonine-protein kinase 14A antibody, STK14A antibody
Tissue Specificity: Expressed in all tissues.
Background: Phosphatidylinositol 4-kinase beta is an enzyme that in humans is encoded by the PI4KB gene. Inositol phospholipids have an important role in intracellular signaling in response to hormones, growth factors and neurotransmitters. Phosphatidylinositol 4-kinase phosphorylates phosphatidylinositol (PI) to phosphatidylinositol-4-phosphate (PIP). In a second step, PIP is further phosphorylated to phosphatidylinositol-4,5-bisphosphate (PIP2), and PIP2 is subsequently hydrolyzed by phospholipase C, producing the two intracellular second messengers, inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG). PI4K230, PI4K92 and PI4K55 are three PI4 kinase isoforms that have been characterized and classified according to their molecular weights of 230, 92 and 55 kD. Previously, PI4 kinases were classified into type II and III enzymes. All isoforms are located on distinct membranes and cellular compartments suggesting various tasks. PI4K230 is located at the endoplasmatic reticulum and outer membranes of mitochondria, PI4K92 at the Golgi apparatus and endoplasmatic reticulum, and PI4K55 at the plasma membrane and endosomes. PI4K230 is predominantly expressed in brain and moderately sensitive to wortmannin as well as specifically and irreversibly inhibited by cyclitol derivatives.
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分子量
- 95 kDa
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基因ID
- 5298
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途径
- Inositol Metabolic Process
抗原
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