EIF3H 抗体 (AA 37-352)
Quick Overview for EIF3H 抗体 (AA 37-352) (ABIN7601501)
抗原
See all EIF3H 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 37-352
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原理
- Anti-EIF3H Antibody Picoband®
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交叉反应 (详细)
- No cross-reactivity with other proteins
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产品特性
- Anti-EIF3H Antibody Picoband® (ABIN7601501). Tested in WB, IHC, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human EIF3H recombinant protein (Position: K37-N352). Human EIF3H shares 98.1% amino acid (aa) sequence identity with both mouse and rat EIF3H.
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亚型
- IgG
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Human, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL
1. Asano, K., Vornlocher, H.-P., Richter-Cook, N. J., Merrick, W. C., Hinnebusch, A. G., Hershey, J. W. B. Structure of cDNAs encoding human eukaryotic initiation factor 3 subunits: possible roles in DNA binding and macromolecular assembly. J. Biol. Chem. 272: 27042-27052, 1997. 2. Choe, J., Lin, S., Zhang, W., Liu, Q., Wang, L., Ramirez-Moya, J., Du, P., Kim, W., Tang, S., Sliz, P., Santisteban, P., George, R. E., Richards, W. G., Wong, K.-K., Locker, N., Slack, F. J., Gregory, R. I. mRNA circularization by METTL3-eIF3h enhances translation and promotes oncogenesis. Nature 561: 556-560, 2018. 3. Kittler, R., Putz, G., Pelletier, L., Poser, I., Heninger, A.-K., Drechsel, D., Fischer, S., Konstantinova, I., Habermann, B., Grabner, H., Yaspo, M.-L., Himmelbauer, H., Korn, B., Neugebauer, K., Pisabarro, M. T., Buchholz, F. An endoribonuclease-prepared siRNA screen in human cells identifies genes essential for cell division. Nature 432: 1036-1040, 2004. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- EIF3H (Eukaryotic Translation Initiation Factor 3 Subunit H (EIF3H))
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别名
- EIF3H
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背景
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Synonyms: EIF3H, EIF3S3, Eukaryotic translation initiation factor 3 subunit H, eIF3h, Eukaryotic translation initiation factor 3 subunit 3, eIF-3-gamma, eIF3 p40 subunit
Background: Enables metal-dependent deubiquitinase activity. Contributes to translation initiation factor activity. Involved in negative regulation of proteasomal ubiquitin-dependent protein catabolic process and translational initiation. Located in extracellular exosome and membrane. Part of eukaryotic translation initiation factor 3 complex. Implicated in breast cancer, prostate cancer, and prostate carcinoma. Biomarker of prostate cancer.
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分子量
- 40 kDa
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基因ID
- 8667
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UniProt
- O15372
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途径
- Ribonucleoprotein Complex Subunit Organization
抗原
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