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RLIM 抗体 (AA 369-621)

This anti-RLIM antibody is a 兔 多克隆 antibody detecting RLIM in WB, ELISA, IHC, IF 和 FACS. Suitable for 人, 小鼠 和 大鼠.
产品编号 ABIN7601490
发货至: 中国

Quick Overview for RLIM 抗体 (AA 369-621) (ABIN7601490)

抗原

See all RLIM 抗体
RLIM (Ring Finger Protein, LIM Domain Interacting (RLIM))

适用

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人, 小鼠, 大鼠

宿主

  • 33
  • 5

克隆类型

  • 36
  • 2
多克隆

标记

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This RLIM antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
  • 抗原表位

    • 15
    • 7
    • 4
    • 2
    • 2
    • 1
    • 1
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    • 1
    • 1
    AA 369-621

    原理

    Anti-RLIM Antibody Picoband®

    产品特性

    Anti-RLIM Antibody Picoband® (ABIN7601490). Tested in WB, IHC, IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    纯化方法

    Immunogen affinity purified.

    免疫原

    E.coli-derived human RLIM recombinant protein (Position: R369-E621). Human RLIM shares 95.4% amino acid (aa) sequence identity with mouse RLIM.
  • 应用备注

    Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
    Immunohistochemistry, 2-5 μg/mL, Human
    Immunofluorescence, 5 μg/mL, Human
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
    ELISA, 0.1-0.5 μg/mL, -
    1. Bach, I., Rodriguez-Esteban, C., Carriere, C., Bhushan, A., Krones, A., Rose, D. W., Glass, C. K., Andersen, B., Belmonte, J. C. I., Rosenfeld, M. G. RLIM inhibits activity of LIM homeodomain transcription factors via recruitment of the histone deacetylase complex. Nature Genet. 22: 394-399, 1999. 2. Frints, S. G. M., Ozanturk, A., Rodriguez Criado, G., Grasshoff, U., de Hoon, B., Field, M., Manouvrier-Hanu, S., Hickey, S. E., Kammoun, M., Gripp, K. W., Bauer, C., Schroeder, C., and 33 others. Pathogenic variants in E3 ubiquitin ligase RLIM/RNF12 lead to a syndromic X-linked intellectual disability and behavior disorder. Molec. Psychiat. 24: 1748-1768, 2019. 3. Gontan, C., Achame, E. M., Demmers, J., Barakat, T. S., Rentmeester, E., van IJcken, W., Grootegoed, J. A., Gribnau, J. RNF12 initiates X-chromosome inactivation by targeting REX1 for degradation. Nature 485: 386-390, 2012.

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    浓度

    500 μg/mL

    缓冲液

    Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

    储存条件

    4 °C,-20 °C

    储存方法

    At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
  • 抗原

    RLIM (Ring Finger Protein, LIM Domain Interacting (RLIM))

    别名

    RLIM

    背景

    E3 ubiquitin-protein ligase RLIM is an enzyme that in humans is encoded by the RLIM gene. The protein encoded by this gene is a RING-H2 zinc finger protein. It has been shown to be an E3 ubiquitin protein ligase that targets LIM domain binding 1 (LDB1/CLIM), and causes proteasome-dependent degradation of LDB1. This protein and LDB1 are co-repressors of LHX1/LIM-1, a homeodomain transcription factor. Multiple alternatively spliced variants, encoding the same protein, have been identified.

    分子量

    69 kDa

    基因ID

    51132
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