PIK3R4 抗体 (AA 36-1339)
Quick Overview for PIK3R4 抗体 (AA 36-1339) (ABIN7601458)
抗原
See all PIK3R4 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 36-1339
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原理
- Anti-PIK3R4 Antibody Picoband®
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交叉反应 （详细）
- No cross reactivity with other proteins.
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产品特性
- Anti-PIK3R4 Antibody Picoband® (ABIN7601458). Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human PIK3R4 recombinant protein (Position: R36-Q1339). Human PIK3R4 shares 95.7% and 95.6% amino acid (aa) sequence identity with mouse and rat PIK3R4, respectively.
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亚型
- IgG
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anti-Phosphoinositide 3-kinase Regulatory Subunit 4 (PIK3R4) (AA 1259-1358) antibody
PIK3R4 适用: 人 ELISA, WB, IF, IHC (p) 宿主: 小鼠 Monoclonal 1B5 unconjugated
anti-Phosphoinositide 3-kinase Regulatory Subunit 4 (PIK3R4) (AA 1259-1358) antibodyPIK3R4 适用: 人 ELISA, WB, RNAi 宿主: 小鼠 Monoclonal 1D4 unconjugated
anti-Phosphoinositide 3-kinase Regulatory Subunit 4 (PIK3R4) (AA 772-906) antibodyPIK3R4 适用: 人 ELISA, IHC, ICC, FACS 宿主: 小鼠 Monoclonal 5H3A11 unconjugated
anti-Phosphoinositide 3-kinase Regulatory Subunit 4 (PIK3R4) antibodyPIK3R4 适用: 人 WB, IF 宿主: 兔 Monoclonal unconjugated
anti-Phosphoinositide 3-kinase Regulatory Subunit 4 (PIK3R4) (AA 1300-1358), (Regulatory Subunit 4) antibodyPIK3R4 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Phosphoinositide 3-kinase Regulatory Subunit 4 (PIK3R4) (AA 772-906) antibodyPIK3R4 适用: 人 ELISA 宿主: 小鼠 Monoclonal 2C11E8 unconjugated
anti-Phosphoinositide 3-kinase Regulatory Subunit 4 (PIK3R4) (AA 1259-1358) antibodyPIK3R4 适用: 人 ELISA, WB 宿主: 小鼠 Monoclonal 2E7 unconjugated
anti-Phosphoinositide 3-kinase Regulatory Subunit 4 (PIK3R4) (AA 1-330) antibodyPIK3R4 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Phosphoinositide 3-kinase Regulatory Subunit 4 (PIK3R4) (AA 360-409) antibodyPIK3R4 适用: 人, 小鼠, 豚鼠, 马, Cow, 犬, Bat WB 宿主: 兔 Polyclonal unconjugated
anti-Phosphoinositide 3-kinase Regulatory Subunit 4 (PIK3R4) (AA 53-83), (N-Term) antibodyPIK3R4 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB1721 unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Gross, M. B. Personal Communication. Baltimore, Md. 5/16/2018. 2. Gstrein, T., Edwards, A., Pristoupilova, A., Leca, I., Breuss, M., Pilat-Carotta, S., Hansen, A. H., Tripathy, R., Traunbauer, A. K., Hochstoeger, T., Rosoklija, G., Repic, M., and 15 others. Mutations in Vps15 perturb neuronal migration in mice and are associated with neurodevelopmental disease in humans. Nature Neurosci. 21: 207-217, 2018. Note: Erratum: Nature Neurosci. 21: 1139 only, 2018. 3. Panaretou, C., Domin, J., Cockcroft, S., Waterfield, M. D. Characterization of p150, an adaptor protein for the human phosphatidylinositol (PtdIns) 3-kinase: substrate presentation by phosphatidylinositol transfer protein to the p150-PtdIns 3-kinase complex. J. Biol. Chem. 272: 2477-2485, 1997. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- PIK3R4 (Phosphoinositide 3-kinase Regulatory Subunit 4 (PIK3R4))
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别名
- PIK3R4
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背景
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Synonyms: 70 kDa ribosomal protein S6 kinase 1 antibody, KS6B1_HUMAN antibody, p70 alpha antibody, P70 beta 1 antibody, p70 ribosomal S6 kinase alpha antibody, p70 ribosomal S6 kinase beta 1 antibody, p70 S6 kinase alpha antibody, P70 S6 Kinase antibody, p70 S6 kinase alpha 1 antibody, p70 S6 kinase alpha 2 antibody, p70 S6K antibody, p70 S6K-alpha antibody, p70 S6KA antibody, p70(S6K) alpha antibody, p70(S6K)-alpha antibody, p70-alpha antibody, p70-S6K 1 antibody, p70-S6K antibody, P70S6K antibody, P70S6K1 antibody, p70S6Kb antibody, PS6K antibody, Ribosomal protein S6 kinase 70 kDa polypeptide 1 antibody, Ribosomal protein S6 kinase beta 1 antibody, Ribosomal protein S6 kinase beta-1 antibody, Ribosomal protein S6 kinase I antibody, RPS6KB1 antibody, S6K antibody, S6K-beta-1 antibody, S6K1 antibody, Serine/threonine kinase 14 alpha antibody, Serine/threonine-protein kinase 14A antibody, STK14A antibody
Tissue Specificity: Expressed in all tissues.
Background: Phosphoinositide 3-kinase regulatory subunit 4, also known as PI3-kinase regulatory subunit 4 or PI3-kinase p150 subunit or phosphoinositide 3-kinase adaptor protein, or VPS15 is an enzyme that in humans is encoded by the PIK3R4 gene. The serine/threonine PI3 kinase regulatory subunit 4 (PIK3R4, Vps15) is the mammalian homologue of the yeast vacuolar protein sorting 15. PIK3R4 regulates the kinase activity of PI3K class III and anchors the kinase to cellular membranes through myristoylation. Recruitment of PI3K class III to the site of early endosome fusion and docking is ly mediated by PIK3R4 binding to the small GTPase Rab5 through its HEAT and WD-40 domains. The PIK3R4/PI3K class III plays a role in late endosome function through PIK3R4 binding to the Rab7 GTPase. In addition to its role in trafficking, the PIK3R4/PI3K class III complex interacts with beclin-1 to play a role during several stages of autophagy. Autophagosome formation is stimulated when Atg14 complexes with PIK3R4, PI3K class III, and beclin-1. The UVRAG protein competes with Atg14 for beclin-1 binding, forming a mutually exclusive complex with PIK3R4, PI3K class III, and beclin-1 that regulates autophagosome maturation. Autophagosome maturation is impaired in the presence of the beclin-1-binding protein Rubicon. Co-expression of PIK3R4 is required for PI3K class III activation and regulation by both beclin-1/UVRAG and by nutrients. Overexpression of PIK3R4 protein has been associated with decreased survival in patients with ovarian tumors, while mutations of the corresponding PIK3R4 gene are associated with metastatic melanoma, suggesting that PIK3R4 functions in cancer.
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分子量
- 150 kDa
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基因ID
- 30849
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UniProt
- Q99570
抗原
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