ELISA, Western Blotting (WB), Flow Cytometry (FACS)
原理
Anti-GLT25D1/COLGALT1 Antibody Picoband®
产品特性
Anti-GLT25D1/COLGALT1 Antibody Picoband® (ABIN7601391). Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
纯化方法
Immunogen affinity purified.
免疫原
E.coli-derived human COLGALT1 recombinant protein (Position: A34-D454). Human GLT25D1/COLGALT1 shares 93.6% amino acid (aa) sequence identity with mouse COLGALT1.
Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/mL, - 1. Baumann, S., Hennet, T. Collagen accumulation in osteosarcoma cells lacking GLT25D1 collagen galactosyltransferase. J. Biol. Chem. 291: 18514-18524, 2016. 2. Hartz, P. A. Personal Communication. Baltimore, Md. June 9, 2017. 3. Liefhebber, J. M. P., Punt, S., Spaan, W. J. M., van Leeuwen, H. C. The human collagen beta(1-O)galactosyltransferase, GLT25D1, is a soluble endoplasmic reticulum localized protein. BMC Cell Biol. 11: 33, 2010. Note: Electronic Article.
限制
仅限研究用
状态
Lyophilized
溶解方式
Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
The protein encoded by this gene is one of two enzymes that transfers galactose moieties to hydroxylysine residues of collagen and mannose binding lectin. This gene is constitutively expressed and encodes a soluble protein that localizes to the endoplasmic reticulum.