ENAH 抗体 (AA 32-551)
Quick Overview for ENAH 抗体 (AA 32-551) (ABIN7601316)
抗原
See all ENAH 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 32-551
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原理
- Anti-ENAH/MENA Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-ENAH/MENA Antibody Picoband® (ABIN7601316). Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human ENAH/MENA recombinant protein (Position: F32-E551).
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亚型
- IgG
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anti-Enabled Homolog (ENAH) (AA 291-390) antibody
ENAH 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3E6 unconjugated
anti-Enabled Homolog (ENAH) (AA 485-589) antibodyENAH 适用: 人 WB, ELISA, Coat 宿主: 小鼠 Monoclonal ENAH-1988 unconjugated
anti-Enabled Homolog (ENAH) (AA 412-591) antibodyENAH 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Enabled Homolog (ENAH) (Internal Region) antibodyENAH 适用: 人 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Enabled Homolog (ENAH) (C-Term) antibodyENAH 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Enabled Homolog (ENAH) antibodyENAH 适用: 人, 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Enabled Homolog (ENAH) (AA 671-720) antibodyENAH 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Enabled Homolog (ENAH) (C-Term) antibodyENAH 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Enabled Homolog (ENAH) (AA 472-521) antibodyENAH 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. Ermekova, K. S., Zambrano, N., Linn, H., Minopoli, G., Gertler, F., Russo, T., Sudol, M. The WW domain of neural protein FE65 interacts with proline-rich motifs in Mena, the mammalian homolog of Drosophila Enabled. J. Biol. Chem. 272: 32869-32877, 1997. 2. Laurent, V., Loisel, T. P., Harbeck, B., Wehman, A., Grobe, L., Jockusch, B. M., Wehland, J., Gertler, F. B., Carlier, M.-F. Role of proteins of the Ena/VASP family in actin-based motility of Listeria monocytogenes. J. Cell Biol. 144: 1245-1258, 1999. 3. Philippar, U., Roussos, E. T., Oser, M., Yamaguchi, H., Kim, H.-D., Giampieri, S., Wang, Y., Goswami, S., Wyckoff, J. B., Lauffenburger, D. A., Sahai, E., Condeelis, J. S., Gertler, F. B. A Mena invasion isoform potentiates EGF-induced carcinoma cell invasion and metastasis. Dev. Cell 15: 813-828, 2008. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- ENAH (Enabled Homolog (ENAH))
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别名
- ENAH
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背景
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Synonyms: Cytoskeleton-associated protein 5, Colonic and hepatic tumor overexpressed gene protein, Ch-TOG, CKAP5, KIAA0097
Tissue Specificity: Expressed in fetal brain. Highly expressed in brain and placenta. Lower levels in heart, liver, thymus, kidney and lung. Located to endothelial cells and neuronal cells of the suprachiasmatic nucleus (SCN). Also detected in endothelial cells of the heart, lung and kidney. In the brain, specifically expressed in the thalamus, hippocampus and amygdala.
Background: Protein enabled homolog is a protein that in humans is encoded by the ENAH gene. This gene encodes a member of the enabled/ vasodilator-stimulated phosphoprotein. Members of this gene family are involved in actin-based motility. This protein is involved in regulating the assembly of actin filaments and modulates cell adhesion and motility. Alternate splice variants of this gene have been correlated with tumor invasiveness in certain tissues and these variants may serve as prognostic markers. A pseudogene of this gene is found on chromosome 3.
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分子量
- 88 kDa
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基因ID
- 55740
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UniProt
- Q8N8S7
抗原
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