PYGL 抗体 (AA 313-804)
Quick Overview for PYGL 抗体 (AA 313-804) (ABIN7601287)
抗原
See all PYGL 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 313-804
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原理
- Anti-PYGL Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-PYGL Antibody Picoband® (ABIN7601287). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human PYGL recombinant protein (Position: K313-K804).
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亚型
- IgG
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anti-phosphorylase, Glycogen, Liver (PYGL) (AA 370-538) antibody
PYGL 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-phosphorylase, Glycogen, Liver (PYGL) (AA 1-280) antibodyPYGL 适用: 人 WB, IHC, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-phosphorylase, Glycogen, Liver (PYGL) (AA 2-846) antibodyPYGL 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-phosphorylase, Glycogen, Liver (PYGL) antibodyPYGL 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-phosphorylase, Glycogen, Liver (PYGL) (AA 1-847) antibodyPYGL 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-phosphorylase, Glycogen, Liver (PYGL) (AA 341-509) antibodyPYGL 适用: 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-phosphorylase, Glycogen, Liver (PYGL) (AA 809-843) antibodyPYGL 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB53470 unconjugated
anti-phosphorylase, Glycogen, Liver (PYGL) (C-Term) antibodyPYGL 适用: 人, 猴 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-phosphorylase, Glycogen, Liver (PYGL) (Internal Region) antibodyPYGL 适用: 人, 犬 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-phosphorylase, Glycogen, Liver (PYGL) (AA 603-750) antibodyPYGL 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Human, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Burwinkel, B., Bakker, H. D., Herschkovitz, E., Moses, S. W., Shin, Y. S., Kilimann, M. W. Mutations in the liver glycogen phosphorylase gene (PYGL) underlying glycogenosis type VI (Hers disease). Am. J. Hum. Genet. 62: 785-791, 1998. 2. Chang, S., Rosenberg, M. J., Morton, H., Francomano, C. A., Biesecker, L. G. Identification of a mutation in liver glycogen phosphorylase in glycogen storage disease type VI. Hum. Molec. Genet. 7: 865-870, 1998. 3. Ercan-Fang, N., Gannon, M. C., Rath, V. L., Treadway, J. L., Taylor, M. R., Nuttall, F. Q. Integrated effects of multiple modulators on human liver glycogen phosphorylase alpha. Am. J. Physiol. Endocr. Metab. 283: E29-E37, 2002. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- PYGL (phosphorylase, Glycogen, Liver (PYGL))
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别名
- PYGL
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背景
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Synonyms: ELAV-like protein 2, ELAV-like neuronal protein 1, Hu-antigen B, HuB, Nervous system-specific RNA-binding protein Hel-N1, ELAVL2, HUB
Tissue Specificity: Brain, neural-specific.
Background: Glycogen phosphorylase, liver form (PYGL), also known as human liver glycogen phosphorylase (HLGP), is an enzyme that in humans is encoded by the PYGL gene on chromosome 14. This gene encodes a homodimeric protein that catalyses the cleavage of alpha-1,4-glucosidic bonds to release glucose-1-phosphate from liver glycogen stores. This protein switches from inactive phosphorylase B to active phosphorylase A by phosphorylation of serine residue 15. Activity of this enzyme is further regulated by multiple allosteric effectors and hormonal controls. Humans have three glycogen phosphorylase genes that encode distinct isozymes that are primarily expressed in liver, brain and muscle, respectively. The liver isozyme serves the glycemic demands of the body in general while the brain and muscle isozymes supply just those tissues. In glycogen storage disease type VI, also known as Hers disease, mutations in liver glycogen phosphorylase inhibit the conversion of glycogen to glucose and results in moderate hypoglycemia, mild ketosis, growth retardation and hepatomegaly. Alternative splicing results in multiple transcript variants encoding different isoforms.
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分子量
- 97 kDa
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基因ID
- 5836
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UniProt
- P06737
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途径
- Carbohydrate Homeostasis, Cellular Glucan Metabolic Process
抗原
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