CD16 抗体 (AA 31-215)
Quick Overview for CD16 抗体 (AA 31-215) (ABIN7601253)
抗原
See all CD16 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 31-215
-
原理
- Anti-CD16/Fcgr3 Antibody
-
交叉反应 (详细)
- No cross-reactivity with other proteins.
-
产品特性
- Anti-CD16/Fcgr3 Antibody (ABIN7601253). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.
-
纯化方法
- Immunogen affinity purified.
-
免疫原
- E.coli-derived mouse CD16/Fcgr3 recombinant protein (Position: A31-T215).
-
亚型
- IgG
-
-
-
-
应用备注
-
Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Mouse
ELISA, 0.1-0.5 μg/mL, -
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
-
浓度
- 500 μg/mL
-
缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
-
储存条件
- 4 °C,-20 °C
-
储存方法
-
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
-
-
- CD16
-
别名
- Fcgr3
-
背景
-
Synonyms: Protein Bop, BH3-only protein, Retrotransposon Gag-like protein 10, RTL10, BOP, C22orf29
Tissue Specificity: Ubiquitously expressed.
Background: Fc receptors bind the most common class of antibody, IgG, are called Fc gamma receptors (FcγR). FcγR is divided into three classes, Fc γ RI (CD64), Fc γ RII (CD32), and Fc γ RIII (CD16). CD16 protein is a multifunctional, low/intermediate affinity receptor, which belongs to the immunoglobulin superfamily. It is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. Mouse CD16 is encoded by a single gene, while, human CD16 is expressed as two distinct forms (CD16a/FcγRIIIa and CD16b/FcγRIIIb) encoded by two different highly homologous genes in a cell type-specific manner. CD16 is involved in phagocytosis, secretion of enzymes, inflammatory mediators, antibody-dependent cellular cytotoxicity (ADCC), and clearance of immune complexes.
-
分子量
- 30 kDa
-
基因ID
- 14131
-
UniProt
- P08508
-
途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
抗原
-