SAP130 抗体 (AA 309-913)
Quick Overview for SAP130 抗体 (AA 309-913) (ABIN7601247)
抗原
See all SAP130 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 309-913
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原理
- Anti-SAP130 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-S Antibody Picoband® (ABIN7601247). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human SAP130 recombinant protein (Position: H309-H913).
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亚型
- IgG
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anti-Sin3A-Associated Protein, 130kDa (SAP130) (N-Term) antibody
SAP130 适用: 人, 大鼠, Cow WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Sin3A-Associated Protein, 130kDa (SAP130) (C-Term) antibodySAP130 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Sin3A-Associated Protein, 130kDa (SAP130) antibodySAP130 适用: 人, 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Sin3A-Associated Protein, 130kDa (SAP130) (AA 819-1048) antibodySAP130 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.25 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Fleischer, T. C., Yun, U. J., Ayer, D. E. Identification and characterization of three new components of the mSin3A corepressor complex. Molec. Cell Biol. 23: 3456-3467, 2003. 2. Metselaar, P. I., Hos, C., Welting, O., Bosch, J. A., Kraneveld, A. D., de Jonge, W. J., Te Velde, A. A. Ambiguity about splicing factor 3b subunit 3 (SF3B3) and Sin3A associated protein 130 (SAP30). Cells 10: 590, 2021. 3. Seifert, L., Werba, G., Tiwari, S., Giao Ly, N. N., Alothman, S., Alqunaibit, D., Avanzi, A., Barilla, R., Daley, D., Greco, S. H., Torres-Hernandez, A., Pergamo, M., and 10 others. The necrosome promotes pancreatic oncogenesis via CXCL1 and Mincle-induced immune suppression. Nature 532: 245-249, 2016. Note: Erratum: Nature 591: E28, 2021. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- SAP130 (Sin3A-Associated Protein, 130kDa (SAP130))
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别名
- SAP130
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背景
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Synonyms: C-C motif chemokine 16, Chemokine CC-4, HCC-4, Chemokine LEC, IL-10-inducible chemokine, LCC-1, Liver-expressed chemokine, Lymphocyte and monocyte chemoattractant, LMC, Monotactin-1, MTN-1, NCC-4, Small-inducible cytokine A16, CCL16, ILINCK, NCC4, SCYA16
Tissue Specificity: Mainly expressed in liver, also found in spleen and thymus. Highly expressed in LPS- and IFN-gamma- activated monocytes, weakly in some lymphocytes, including natural killer cells, gamma-delta T-cells, and some T-cell clones.
Background: Histone deacetylase complex subunit SAP130 is an enzyme that in humans is encoded by the SAP130 gene. This gene encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair.
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分子量
- 130 kDa
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基因ID
- 79595
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UniProt
- Q9H0E3
抗原
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