CRYAB 抗体 (AA 30-151)
Quick Overview for CRYAB 抗体 (AA 30-151) (ABIN7601209)
抗原
See all CRYAB 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 30-151
-
原理
- Anti-Alpha B Crystallin/CRYAB Antibody Picoband®
-
交叉反应 （详细）
- No cross-reactivity with other proteins.
-
产品特性
- Anti-Alpha B Crystallin/CRYAB Antibody Picoband® (ABIN7601209). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
-
纯化方法
- Immunogen affinity purified.
-
免疫原
- E.coli-derived human Alpha B Crystallin/CRYAB recombinant protein (Position: E30-Q151).
-
亚型
- IgG
-
-
anti-Crystallin, alpha B (CRYAB) (AA 1-175) antibody
CRYAB 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Crystallin, alpha B (CRYAB) antibodyCRYAB 适用: 人 WB, IHC, IHC (p), IP, FACS 宿主: 小鼠 Monoclonal 6G5 unconjugated
anti-Crystallin, alpha B (CRYAB) antibodyCRYAB 适用: 人, 大鼠 WB, IHC, StM 宿主: 小鼠 Monoclonal CPTC-CRYAB-1 unconjugated
anti-Crystallin, alpha B (CRYAB) (AA 1-175) antibodyCRYAB 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Crystallin, alpha B (CRYAB) antibodyCRYAB 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Crystallin, alpha B (CRYAB) (AA 1-175) antibodyCRYAB 适用: 人 ELISA, IHC 宿主: 小鼠 Monoclonal 1D11C6E6 unconjugated
anti-Crystallin, alpha B (CRYAB) antibody (HRP)CRYAB 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal HRP
anti-Crystallin, alpha B (CRYAB) antibody (FITC)CRYAB 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal FITC
anti-Crystallin, alpha B (CRYAB) antibody (HRP)CRYAB 适用: 人 WB, ELISA, IHC, IF, ICC 宿主: 小鼠 Monoclonal 3A10-C9 HRP
anti-Crystallin, alpha B (CRYAB) antibody (Biotin)CRYAB 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal Biotin
-
-
应用备注
-
Western blot, 0.1-0.25 μg/mL, Mouse, Rat, Monkey
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Berry, V., Francis, P., Reddy, M. A., Collyer, D., Vithana, E., MacKay, I., Dawson, G., Carey, A. H., Moore, A., Bhattacharya, S. S., Quinlan, R. A. Alpha-B crystallin gene (CRYAB) mutation causes dominant congenital posterior polar cataract in humans. Am. J. Hum. Genet. 69: 1141-1145, 2001. 2. Brakenhoff, R. H., Geurts van Kessel, A. H. M., Oldenburg, M., Wijnen, J. T., Bloemendal, H., Meera Khan, P., Schoenmakers, J. G. G. Human alpha-B-crystallin (CRYA2) gene mapped to chromosome 11q12-q23. Hum. Genet. 85: 237-240, 1990. 3. Chavez Zobel, A. T., Loranger, A., Marceau, N., Theriault, J. R., Lambert, H., Landry, J. Distinct chaperone mechanisms can delay the formation of aggresomes by the myopathy-causing R120G alpha-B-crystallin mutant. Hum. Molec. Genet. 12: 1609-1620, 2003. -
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
-
浓度
- 500 μg/mL
-
缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
-
Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
-
-
- CRYAB (Crystallin, alpha B (CRYAB))
-
别名
- CRYAB
-
背景
-
Synonyms: Calpain-2 catalytic subunit,3.4.22.53,Calcium-activated neutral proteinase 2,CANP 2,Calpain M-type,Calpain large polypeptide L2,Calpain-2 large subunit,Millimolar-calpain,M-calpain,CAPN2,CANPL2,
Tissue Specificity: Ubiquitous.
Background: Alpha-crystallin B chain is a protein that in humans is encoded by the CRYAB gene. Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone, instead they hold them in large soluble aggregates. These heterogeneous aggregates consist of 30-40 subunits, the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed, alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases, a missense mutation cosegregated in a family with a desmin-related myopathy. Alternative splicing results in multiple transcript variants.
-
分子量
- 20 kDa
-
基因ID
- 1410
-
UniProt
- P02511
抗原
-
