DBF4 抗体 (AA 295-665)
Quick Overview for DBF4 抗体 (AA 295-665) (ABIN7601162)
抗原
See all DBF4 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 295-665
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原理
- Anti-DBF4 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-DBF4 Antibody Picoband® (ABIN7601162). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human DBF4 recombinant protein (Position:Y295-S665).
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亚型
- IgG
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anti-DBF4 Homolog (DBF4) (AA 2-98) antibody
DBF4 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 6G9 unconjugated
anti-DBF4 Homolog (DBF4) (AA 101-281) antibodyDBF4 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-DBF4 Homolog (DBF4) (AA 2-98) antibodyDBF4 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-DBF4 Homolog (DBF4) (Internal Region) antibodyDBF4 适用: 人, 小鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-DBF4 Homolog (DBF4) (N-Term) antibodyDBF4 适用: 人, Cow, Pig WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-DBF4 Homolog (DBF4) (N-Term) antibodyDBF4 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-DBF4 Homolog (DBF4) (Internal Region) antibodyVerified DBF4 适用: 小鼠, 大鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-DBF4 Homolog (DBF4) (N-Term) antibodyDBF4 适用: 人, 小鼠, 猴 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-DBF4 Homolog (DBF4) (AA 1-674) antibodyDBF4 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry(Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Brott, B. K., Sokol, S. Y. A vertebrate homolog of the cell cycle regulator Dbf4 is an inhibitor of Wnt signaling required for heart development. Dev. Cell 8: 703-715, 2005. Brott, B. K., Sokol, S. Y. A vertebrate homolog of the cell cycle regulator Dbf4 is an inhibitor of Wnt signaling required for heart development. Dev. Cell 8: 703-715, 2005. 2. Lepke, M., Putter, V., Staib, C., Kneissl, M., Berger, C., Hoehn, K., Nanda, I., Schmid, M., Grummt, F. Identification, characterization and chromosomal localization of the cognate human and murine DBF4 genes. Molec. Gen. Genet. 262: 220-229, 1999. Lepke, M., Putter, V., Staib, C., Kneissl, M., Berger, C., Hoehn, K., Nanda, I., Schmid, M., Grummt, F. Identification, characterization and chromosomal localization of the cognate human and murine DBF4 genes. Molec. Gen. Genet. 262: 220-229, 1999. 3. Zegerman, P., Diffley, J. F. X. Checkpoint-dependent inhibition of DNA replication initiation by Sld3 and Dbf4 phosphorylation. Nature 467: 474-478, 2010. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- DBF4 (DBF4 Homolog (DBF4))
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别名
- DBF4
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背景
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Synonyms: Metabotropic glutamate receptor 5, mGluR5, GRM5, GPRC1E, MGLUR5
Tissue Specificity: Isoform 1 and isoform 2 are detected in bone marrow cells, spermatogonia and spermatocytes, but not in round spermatids, elongating spermatids and spermatozoa. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa, but not in spermatogonia and spermatocytes (at protein level). Isoform 1 is widely expressed and detected in fetal liver and bone marrow. Isoform 3 is detected in bone marrow cells enriched in hematopoietic stem cells.
Background: Using both gain- and loss-of-function experiments, it was found that Xenopus Dbf4 inhibited canonical Wnt signaling. Depletion of endogenous Dbf4 did not disturb gastrulation movements or early organizer genes, but resulted in embryos with morphologically defective heart and eyes and suppressed cardiac markers. The function of Dbf4 in heart development appeared to be independent of its role in the cell cycle. Dbf4 interacted physically and functionally with Frodo, a context-dependent regulator of Wnt signaling.
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分子量
- 80 kDa
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基因ID
- 10926
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途径
- Mitotic G1-G1/S Phases, DNA Replication
抗原
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