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DBF4 抗体 (AA 295-665)

The 兔 多克隆 anti-DBF4 antibody (ABIN7601162) specifically detects DBF4 in WB, ELISA, IF, ICC 和 FACS. The antibody is reactive with 人, 小鼠 和 大鼠 samples.
产品编号 ABIN7601162
发货至: 中国
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Quick Overview for DBF4 抗体 (AA 295-665) (ABIN7601162)

抗原

See all DBF4 抗体
DBF4 (DBF4 Homolog (DBF4))

适用

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人, 小鼠, 大鼠

宿主

  • 45
  • 4
  • 1

克隆类型

  • 42
  • 8
多克隆

标记

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  • 1
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This DBF4 antibody is un-conjugated

应用范围

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  • 18
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Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)
  • 抗原表位

    • 15
    • 5
    • 5
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 295-665

    原理

    Anti-DBF4 Antibody

    交叉反应 (详细)

    No cross-reactivity with other proteins.

    产品特性

    Anti-DBF4 Antibody (ABIN7601162). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

    纯化方法

    Immunogen affinity purified.

    免疫原

    E.coli-derived human DBF4 recombinant protein (Position:Y295-S665).

    亚型

    IgG
  • 应用备注

    Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
    Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
    Flow Cytometry(Fixed), 1-3 μg/1x106 cells, Human
    ELISA, 0.1-0.5 μg/mL, -

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    浓度

    500 μg/mL

    缓冲液

    Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

    储存条件

    4 °C,-20 °C

    储存方法

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • 抗原

    DBF4 (DBF4 Homolog (DBF4))

    别名

    DBF4

    背景

    Synonyms: Metabotropic glutamate receptor 5, mGluR5, GRM5, GPRC1E, MGLUR5

    Tissue Specificity: Isoform 1 and isoform 2 are detected in bone marrow cells, spermatogonia and spermatocytes, but not in round spermatids, elongating spermatids and spermatozoa. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa, but not in spermatogonia and spermatocytes (at protein level). Isoform 1 is widely expressed and detected in fetal liver and bone marrow. Isoform 3 is detected in bone marrow cells enriched in hematopoietic stem cells.

    Background: Using both gain- and loss-of-function experiments, it was found that Xenopus Dbf4 inhibited canonical Wnt signaling. Depletion of endogenous Dbf4 did not disturb gastrulation movements or early organizer genes, but resulted in embryos with morphologically defective heart and eyes and suppressed cardiac markers. The function of Dbf4 in heart development appeared to be independent of its role in the cell cycle. Dbf4 interacted physically and functionally with Frodo, a context-dependent regulator of Wnt signaling.

    分子量

    80 kDa

    基因ID

    10926

    途径

    Mitotic G1-G1/S Phases, DNA Replication
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