HIF3A 抗体 (AA 29-480)
Quick Overview for HIF3A 抗体 (AA 29-480) (ABIN7601137)
抗原
See all HIF3A 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 29-480
-
原理
- Anti-HIF3/HIF3A Antibody Picoband®
-
交叉反应 （详细）
- No cross-reactivity with other proteins.
-
产品特性
- Anti-HIF3/HIF3A Antibody Picoband® (ABIN7601137). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
-
纯化方法
- Immunogen affinity purified.
-
免疫原
- E.coli-derived human HIF3/HIF3A recombinant protein (Position: Q29-Q480).
-
亚型
- IgG
-
-
anti-Hypoxia Inducible Factor 3, alpha Subunit (HIF3A) antibody
HIF3A 适用: 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Hypoxia Inducible Factor 3, alpha Subunit (HIF3A) (AA 581-592) antibodyHIF3A 适用: 人, 小鼠 WB, ELISA, IHC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Hypoxia Inducible Factor 3, alpha Subunit (HIF3A) (AA 516-669) antibodyHIF3A 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Hypoxia Inducible Factor 3, alpha Subunit (HIF3A) (AA 1-669) antibodyHIF3A 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Hypoxia Inducible Factor 3, alpha Subunit (HIF3A) (AA 355-669) antibodyHIF3A 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 4C2 unconjugated
anti-Hypoxia Inducible Factor 3, alpha Subunit (HIF3A) (C-Term) antibodyHIF3A 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Hypoxia Inducible Factor 3, alpha Subunit (HIF3A) (C-Term) antibodyHIF3A 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Hypoxia Inducible Factor 3, alpha Subunit (HIF3A) (N-Term) antibodyVerified HIF3A 适用: 人 ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Hypoxia Inducible Factor 3, alpha Subunit (HIF3A) (AA 1-363) antibodyHIF3A 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Hypoxia Inducible Factor 3, alpha Subunit (HIF3A) (Internal Region) antibodyHIF3A 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Hara, S., Hamada, J., Kobayashi, C., Kondo, Y., Imura, N. Expression and characterization of hypoxia-inducible factor (HIF)-3-alpha in human kidney: suppression of HIF-mediated gene expression by HIF-3-alpha. Biochem. Biophys. Res. Commun. 287: 808-813, 2001. 2. Maynard, M. A., Qi, H., Chung, J., Lee, E. H. L., Kondo, Y., Hara, S., Conaway, R. C., Conaway, J. W., Ohh, M. Multiple splice variants of the human HIF-3-alpha locus are targets of the von Hippel-Lindau E3 ubiquitin ligase complex. J. Biol. Chem. 278: 11032-11040, 2003. -
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
-
浓度
- 500 μg/mL
-
缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
-
Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
-
-
- HIF3A (Hypoxia Inducible Factor 3, alpha Subunit (HIF3A))
-
别名
- HIF3A
-
背景
-
Synonyms: "Hypoxia-inducible factor 3-alpha, HIF-3-alpha, HIF3-alpha, Basic-helix-loop-helix-PAS protein MOP7, Class E basic helix-loop-helix protein 17, bHLHe17, HIF3-alpha-1, Inhibitory PAS domain protein, IPAS, Member of PAS protein 7, PAS domain-containing protein 7, HIF3A, BHLHE17, MOP7, PASD7
Tissue Specificity: Expressed in vascular cells (at protein level). Expressed in kidney. Expressed in lung epithelial cells. Expressed in endothelial cells (venous and arterial cells from umbilical cord and aortic endothelial cells) and in vascular smooth muscle cells (aorta). Strongly expressed in the heart, placenta, and skeletal muscle, whereas a weak expression profile was found in the lung, liver, and kidney. Expressed weakly in cell renal cell carcinoma (CC-RCC) compared to normal renal cells. Expression is down-regulated in numerous kidney tumor cells compared to non tumor kidney tissues. Isoform 2 is expressed in heart, placenta, lung, liver, skeletal muscle and pancreas and in numerous cancer cell lines. Isoform 3 and isoform 4 are weakly expressed in heart, placenta, lung, liver, skeletal muscle and pancreas. Isoform 4 is expressed in fetal tissues, such as heart, brain, thymus, lung, liver, skeletal kidney and spleen. Isoform 3 is weakly expressed in fetal tissues, such as liver and kidney.
Background: Hypoxia-inducible factor 3 alpha, also called MOP7, is a protein that in humans is encoded by the HIF3A gene. HIF3A is mapped to 19q13.32. This gene involved in adaptive response to hypoxia. HIF3A suppresses hypoxia-inducible expression of HIF1A and EPAS1. It binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. The complex HIF3A-ARNT activates the transcription of reporter genes driven by HRE. This gene functions as an inhibitor of angiogenesis in the cornea.
-
分子量
- 72 kDa
-
基因ID
- 64344
-
UniProt
- Q9Y2N7
-
途径
- Warburg Effect
抗原
-
